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Species: Homo sapiens
Genetic Insert: Surface-HaloTag-based EXSISERS
Vector Backbone Description: Vector Backbone:pBlueScript-KS II (+); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34083785
Comments: HMGU has filed a patent (WO2020161236A1 “Detection of splice events of a gene of interest”) disclosing the capabilities of EXSISERS, and retains ownership rights to EXSISERS Material.
Proper citation: RRID:Addgene_177869 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting SDHA
Vector Backbone Description: Vector Backbone:pLentiCRISPR v1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34855504
Proper citation: RRID:Addgene_177980 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting COX4
Vector Backbone Description: Vector Backbone:pLentiCRISPR v1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34855504
Proper citation: RRID:Addgene_177983 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting UQCRC2
Vector Backbone Description: Vector Backbone:pLentiCRISPR v1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34855504
Proper citation: RRID:Addgene_177982 Copy
Species: C. albicans (yeast)
Genetic Insert: AOX
Vector Backbone Description: Vector Backbone:pCW57.1-Luciferase; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34855504
Proper citation: RRID:Addgene_177984 Copy
Species: Homo sapiens
Genetic Insert: LDL receptor related protein associated protein 1
Vector Backbone Description: Backbone Marker:Ryan Hibbs; Backbone Size:7427; Vector Backbone:pEZT-BM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34529485
Proper citation: RRID:Addgene_177986 Copy
Species: Homo sapiens
Genetic Insert: UQCRC2
Vector Backbone Description: Vector Backbone:pCW57.1 N-term GFP tTA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34855504
Proper citation: RRID:Addgene_177978 Copy
Species: Homo sapiens
Genetic Insert: SDHB
Vector Backbone Description: Vector Backbone:pCW57.1 N-term GFP tTA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34855504
Proper citation: RRID:Addgene_177977 Copy
Species: Homo sapiens
Genetic Insert: SDHA
Vector Backbone Description: Vector Backbone:pCW57.1 N-term GFP tTA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34855504
Proper citation: RRID:Addgene_177976 Copy
Species: Aquorea victoria
Genetic Insert: Rosa26 mT/mG
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17868096
Comments: See the 'Notes' section for the annotated GenBank file. Please note that some portions of the sequence (e.g., Rosa26 targeting arms and CA promoter) may not be entirely accurate.
Proper citation: RRID:Addgene_17787 Copy
Species: P. putida
Genetic Insert: Cytochrome P450cam
Vector Backbone Description: Backbone Size:3939; Vector Backbone:pEMBL8+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3003058
Proper citation: RRID:Addgene_17788 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27323850
Comments: Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information.
Proper citation: RRID:Addgene_177925 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27323850
Comments: Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information.
Proper citation: RRID:Addgene_177924 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27323850
Comments: Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information.
Proper citation: RRID:Addgene_177928 Copy
Species: Synthetic
Genetic Insert: iPAK4
Vector Backbone Description: Vector Backbone:pLenti cFos; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593408
Proper citation: RRID:Addgene_177883 Copy
Species: Synthetic
Genetic Insert: Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
Vector Backbone Description: Vector Backbone:pAV-U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34937909
Comments: Addgene comment: Please note that the Addgene verified sequence differs from the depositor's original plasmid map and .gb file. Please refer to the Addgene sequence for the most accurate results. The depositor's map is still shown since it contains useful annotations.
Proper citation: RRID:Addgene_177919 Copy
Species: Synthetic
Genetic Insert: Squash aptamer
Vector Backbone Description: Backbone Size:6151; Vector Backbone:pAV-U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34937909
Proper citation: RRID:Addgene_177913 Copy
Species: Synthetic
Genetic Insert: Tornado-Squash-SAM-sensor 4-2
Vector Backbone Description: Backbone Size:6150; Vector Backbone:pAV-U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34937909
Proper citation: RRID:Addgene_177916 Copy
Species: Synthetic
Genetic Insert: Tornado-Squash
Vector Backbone Description: Backbone Size:6151; Vector Backbone:pAV-U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34937909
Proper citation: RRID:Addgene_177915 Copy
Species: Homo sapiens
Genetic Insert: human CD63
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35594496
Proper citation: RRID:Addgene_177910 Copy
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