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Species: Streptococcus pyogenes
Genetic Insert: SrtA(strep)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22348280
Proper citation: RRID:Addgene_50033 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50275 Copy
Species: Streptococcus pyogenes
Genetic Insert: HA-SrtA(strep)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22348280
Proper citation: RRID:Addgene_50032 Copy
Species: Other
Genetic Insert: promoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50274 Copy
Species: Other
Genetic Insert: Promoter + 5'UTR, ocs, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50273 Copy
Species: Other
Genetic Insert: promoter, cab2 (AT1g29930, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50279 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter + 5' UTR, LHB1B1 (AT2g34430, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50278 Copy
Species: HPV
Genetic Insert: HPV 16 E6no*
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pNCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Comments: *The V42L mutation prevents the internal splicing of the DNA so only the "no *" (unspliced form) of 18E6 is produced.
Proper citation: RRID:Addgene_47706 Copy
Species: Synthetic
Genetic Insert: Codon-optimised MSP1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47709 Copy
Species: Other
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Comments: Gene was obtained from collaborator Andor Kiss, Miami University of Ohio.
Proper citation: RRID:Addgene_47666 Copy
Species: Danio rerio
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47664 Copy
Species: Homo sapiens
Genetic Insert: Olfactomedin-like domain with V426F mutation
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6645; Vector Backbone:pmalC4X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21612213
Comments: The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
Proper citation: RRID:Addgene_47702 Copy
Species: Homo sapiens
Genetic Insert: Olfactomedin-like domain with I499F mutation
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6645; Vector Backbone:pmalC4X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21612213
Comments: The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
Proper citation: RRID:Addgene_47703 Copy
Species: Other
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47667 Copy
Genetic Insert: esaI-term
Vector Backbone Description: Backbone Size:4511; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23879176
Comments: esaI is 636 bp and indicated as lowercase letters in the partial sequence (between BamHI and XhoI sites). See pAC-EsaR for lac promoter and esaR sequence information.
Proper citation: RRID:Addgene_47660 Copy
Genetic Insert: PesaR-D
Vector Backbone Description: Backbone Size:9360; Vector Backbone:pCS-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23879176
Comments: PesaR-D (275 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pCS-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pCS-PesaRlux for full plasmid backbone sequence and plasmid map.
Proper citation: RRID:Addgene_47653 Copy
Genetic Insert: PesaR-C
Vector Backbone Description: Backbone Size:8891; Vector Backbone:pET-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-C (274 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pET-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pET-PesaRlux for more details and plasmid map.
Proper citation: RRID:Addgene_47658 Copy
Genetic Insert: PesaR-D
Vector Backbone Description: Backbone Size:8891; Vector Backbone:pET-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-D (275 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pET-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pET-PesaRlux for more details and plasmid map.
Proper citation: RRID:Addgene_47657 Copy
Genetic Insert: esaR-I70V/D91G
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-I70V/D91G (750 bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47644 Copy
Genetic Insert: esaR-I70V/D91G/V220A
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-I70V/D91G/V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47647 Copy
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