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Species: Other
Genetic Insert: NatMx3
Vector Backbone Description: Vector Backbone:pRSII426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is similar to pML104 except that the URA3 marker has been switched for NatMx3 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
Proper citation: RRID:Addgene_83477 Copy
Species: Other
Genetic Insert: KanMX4
Vector Backbone Description: Vector Backbone:pRSII426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is similar to pML104 except that the URA3 marker has been switched for KanMX4 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
Proper citation: RRID:Addgene_83476 Copy
Species: Other
Genetic Insert: WingGFP-tan
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM T-easy; Vector Types:Bacterial Expression, Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27494619
Comments: To be used as a selectable marker in two-stage CRISPR-mediated allele replacement strategies as described in Lamb, et al 2016. This donor contains a selectable green fluorescent protein driven by an enhancer from the D. melanogaster yellow gene that can be screened in the developing pupal wing. The current insert contains homology arms targeting the D. americana tan gene, which can be replaced with homology arms targeting the experimenter's locus of choice.
Construct contains:
5’ homology arm
5’ target PAM
Unique portion of 5’ target site for reporter excision (restriction sites: BglII, BsiWI, Acc65I)
Pupal wing enhancer from D. melanogaster yellow gene
EGFP with Hsp70 promoter and SV40 polyadenylation signal
Unique portion of 3’ target site for reporter excision (restriction sites: NarI, Bsu36I, ClaI)
3’ homology arm
3’ target PAM
Restriction sites within pGEM backbone for homology arm removal: 5’ end – ApaI, SphI; 3’ end – SbfI, MluI
Proper citation: RRID:Addgene_83473 Copy
Species: Other
Genetic Insert: HphMX4
Vector Backbone Description: Vector Backbone:pRSII426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is similar to pML104 except that the URA3 marker has been switched for hphMX4 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.
Proper citation: RRID:Addgene_83475 Copy
Species: Other
Genetic Insert: 3XP3.RFP-tan
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM T-easy; Vector Types:Bacterial Expression, Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27494619
Comments: To be used as a selectable marker in two-stage CRISPR-mediated allele replacement strategies as described in Lamb, et al 2016. This donor contains a selectable red fluorescent protein maker that can be screened in the adult eye. The current insert contains homology arms targeting the D. americana tan gene, which can be replaced with homology arms targeting the experimenter's locus of choice.
Construct contains:
5’ homology arm
5’ target PAM
Unique portion of 5’ target site for reporter excision (restriction sites: BglII, BsiWI, Acc65I)
3XP3 eye-expression Promoter
RFP with α-tubulin 3’UTR
Unique portion of 3’ target site for reporter excision (restriction sites: NarI, Bsu36I, ClaI)
3’ homology arm
3’ target PAM
Restriction sites for homology arm removal: 5’ end – ApaI, NcoI; 3’ end – SexAI, MluI
Proper citation: RRID:Addgene_83474 Copy
Species: Other
Genetic Insert: transposase 5
Vector Backbone Description: Vector Backbone:pNIC-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_79107 Copy
Species: Other
Genetic Insert: 583.m05422
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26092); Backbone Size:7737; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please see http://www.thesgc.org/structures/5J3U for more information.
Proper citation: RRID:Addgene_79525 Copy
Species: Other
Genetic Insert: sHRPaNRX3beta
Vector Backbone Description: Vector Backbone:pXL-BacII; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27240195
Proper citation: RRID:Addgene_79910 Copy
Species: Other
Genetic Insert: Zeocin-t2A-sHRPbNRX3beta
Vector Backbone Description: Vector Backbone:pXL-BacII; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27240195
Proper citation: RRID:Addgene_79913 Copy
Species: Other
Genetic Insert: OsTIR1
Vector Backbone Description: Backbone Size:4958; Vector Backbone:pBabe; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: The osTIR1-9Myc was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
Proper citation: RRID:Addgene_80073 Copy
Species: Other
Genetic Insert: random shRNA
Vector Backbone Description: Backbone Marker:Dr. John Rossi, Beckman Research Institute of the City of Hope, CA; Vector Backbone:Banshee; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80145 Copy
Species: Other
Genetic Insert: OsYSA-gRNA01
Vector Backbone Description: Vector Backbone:pTX172; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Proper citation: RRID:Addgene_89264 Copy
Species: Other
Genetic Insert: OsPDS-gRNA01
Vector Backbone Description: Vector Backbone:pTX172; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Proper citation: RRID:Addgene_89262 Copy
Species: Other
Genetic Insert: OsYSA-gRNA01 and OsYSA-gRNA02
Vector Backbone Description: Vector Backbone:pTX172; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Proper citation: RRID:Addgene_89268 Copy
Species: Other
Genetic Insert: OsYSA-gRNA02
Vector Backbone Description: Vector Backbone:pTX172; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Proper citation: RRID:Addgene_89265 Copy
Species: Other
Genetic Insert: OsPDS-gRNA01 and OsPDS-gRNA02
Vector Backbone Description: Vector Backbone:pTX172; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27212389
Proper citation: RRID:Addgene_89269 Copy
Species: Other
Genetic Insert: TaCas9
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27943461
Comments: Entry vector with TaCas9-nos terminator
Proper citation: RRID:Addgene_89282 Copy
Species: Other
Genetic Insert: Channelrhodopsin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4548; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27525487
Proper citation: RRID:Addgene_89570 Copy
Species: Other
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Luigi Naldini, PMID 9765382; Backbone Size:8499; Vector Backbone:CCLc; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25702874
Proper citation: RRID:Addgene_89608 Copy
Species: Other
Genetic Insert: CRISPR array
Vector Backbone Description: Backbone Marker:PMID:26013814; Vector Backbone:pCDF-casBCDE; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:27738137
Proper citation: RRID:Addgene_89728 Copy
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