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Species: Synthetic
Genetic Insert: PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
Vector Backbone Description: Backbone Size:2689; Vector Backbone:pVH1002 (pUC57-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169605 Copy
Species: Synthetic
Genetic Insert: Taq DNA Ligase
Vector Backbone Description: Vector Backbone:pATetO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34061896
Proper citation: RRID:Addgene_178798 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:RV-Syn; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_185700 Copy
Species: Synthetic
Genetic Insert: Mut2-RS
Vector Backbone Description: Vector Backbone:pEvol; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33350556
Proper citation: RRID:Addgene_182882 Copy
Species: Synthetic
Genetic Insert: Mut1-RS
Vector Backbone Description: Vector Backbone:pEvol; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33350556
Proper citation: RRID:Addgene_182881 Copy
Species: Synthetic
Genetic Insert: PRPBSA-driven PuroR expression
Vector Backbone Description: Backbone Size:3105; Vector Backbone:pTS1107 (pUC57-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169639 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Vector Backbone:pGRG36; Vector Types:Bacterial Expression, miniTn7 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118559 Copy
Species: Synthetic
Genetic Insert: SBFP2-anti-AlfaTag nanobody
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pColdII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34089703
Proper citation: RRID:Addgene_171826 Copy
Species: Synthetic
Genetic Insert: PPGK-driven expression of secreted Nluc and mTagBFP2
Vector Backbone Description: Backbone Size:2689; Vector Backbone:pVH1002 (pUC57-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169619 Copy
Species: Synthetic
Genetic Insert: CF-mCherry
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35179286
Proper citation: RRID:Addgene_183166 Copy
Species: Synthetic
Genetic Insert: humanized ChR2-H134R (partial)
Vector Backbone Description: Backbone Marker:Edward Boyden; Backbone Size:6150; Vector Backbone:AAVrg-CAG-tdTomato-WPRE-SV40 (Addgene, Plasmid #59462); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35296859
Proper citation: RRID:Addgene_180784 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
Vector Backbone Description: Backbone Size:6311; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35990749
Comments: This plasmid is based on the pUCmini-iCAP-PHP.eB plasmid. Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_184592 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-eDHFR(69K6)
Vector Backbone Description: Backbone Marker:Dr. Hiroyuki Miyoshi (RIKEN, Japan); Backbone Size:10345; Vector Backbone:pCSII-EF-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35835118
Comments: The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178853 Copy
Species: Synthetic
Genetic Insert: MTOR
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183311 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:7456; Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35294338
Proper citation: RRID:Addgene_187482 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:lenti_Cas9; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35387989
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.03.974923v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_183208 Copy
Species: Synthetic
Genetic Insert: sfGFP-GB1-scAB
Vector Backbone Description: Backbone Size:5365; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185794 Copy
Species: Synthetic
Genetic Insert: pcp-12xSunTag
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185799 Copy
Species: Synthetic
Genetic Insert: sfGFP, tdTomato
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Dre/Rox; Bacterial Resistance:Ampicillin
Comments: cre → sfGFP expression. dre → tdTomato expression.
Expression of sfGFP and tdTomato are independent of each other. "OR."
Actual mouse was not generated.
The EMCV IRES fragment from Dr. Peter Mombaerts is the same EMCV IRES used in the Id1-IRES-creERT2 knock-in mouse line (http://www.informatics.jax.org/allele/MGI:4366863).
Proper citation: RRID:Addgene_185966 Copy
Species: Synthetic
Genetic Insert: sfGFP, tdTomato
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Dre/Rox; Bacterial Resistance:Ampicillin
Comments: cre → sfGFP expression. cre and dre → sfGFP and tdTomato expression. dre only (no cre) → no sfGFP nor tdTomato expression.
The actual mouse did not have any abnormalities.
Proper citation: RRID:Addgene_185965 Copy
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