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Species: Homo sapiens
Genetic Insert: TS2 sgRNA
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26480473
Proper citation: RRID:Addgene_69234 Copy
Species: Homo sapiens
Genetic Insert: CLDN18
Vector Backbone Description: Backbone Marker:cell biolabs; Backbone Size:5732; Vector Backbone:pMXs-Puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26146084
Proper citation: RRID:Addgene_69466 Copy
Species: Homo sapiens
Genetic Insert: MLL3-PRKAG2
Vector Backbone Description: Backbone Marker:cell biolabs; Backbone Size:5604; Vector Backbone:pMXs-Puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26146084
Proper citation: RRID:Addgene_69473 Copy
Species: Homo sapiens
Genetic Insert: Rac1
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16472675
Comments: Rac1 has a mutation, G15A, which makes it nucleotide free when expressed in bacteria, which can be used GEF pulldowns.
Proper citation: RRID:Addgene_69355 Copy
Species: Homo sapiens
Genetic Insert: MDR1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5598; Vector Backbone:pGL2 Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9632821
Proper citation: RRID:Addgene_69483 Copy
Species: Homo sapiens
Genetic Insert: 53BP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8003; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25984718
Comments: Note: This construct contains amino acids 1220-1709 of 53BP1 followed by the short peptide VNDLDNSTG-STOP. This additional peptide does not affect reporter activity, and the plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_69531 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24006363
Comments: The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.
Proper citation: RRID:Addgene_69579 Copy
Species: Homo sapiens
Genetic Insert: L1PA1
Vector Backbone Description: Backbone Size:3530; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The blast rescue cassette was commercially synthesized and cloned into the EcoRI-NotI sites of L1PA1CHmneo to swap the mneo cassette with the blast rescue cassette sequence
Proper citation: RRID:Addgene_69611 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25344068
Comments: The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3. This is tagged with the N-terminus of a split mVenus.
Proper citation: RRID:Addgene_69582 Copy
Species: Homo sapiens
Genetic Insert: p53-L344P
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24006363
Comments: This mutant will not dimerize nor tetramerize. The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.
Proper citation: RRID:Addgene_69581 Copy
Species: Homo sapiens
Genetic Insert: p53-L344A
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24006363
Comments: This mutant will dimerize, but not tetramerize. The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.
Proper citation: RRID:Addgene_69580 Copy
Species: Homo sapiens
Genetic Insert: p53-L344P
Vector Backbone Description: Backbone Size:7429; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25344068
Comments: This mutant will not dimerize nor tetramerize. This p53 is tagged with the N-term of a split mVenus. The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.
Proper citation: RRID:Addgene_69586 Copy
Species: Homo sapiens
Genetic Insert: RPS27A
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3626; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26235645
Comments: Original sequence encoded human RPS27a. This sequence contains a point mutation (A133G) in order to encode the mouse protein.
Proper citation: RRID:Addgene_69559 Copy
Species: Homo sapiens
Genetic Insert: CYP2C9
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCW; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Modification of CYP2C9 for bacterial expression as per Barnes et al. PNAS 88:5597, 1991 [PMID: 1829523]: First 8 amino acids of CYP2C cDNAs replaced with those of bovine 17α hydroxylase (MALLLAVF) to optimize bacterial expression.
Proper citation: RRID:Addgene_69554 Copy
Species: Homo sapiens
Genetic Insert: CYP2C19
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCW; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Modification of CYP2C19 for bacterial expression as per Barnes et al. PNAS 88:5597, 1991 [PMID: 1829523]: First 8 amino acids of CYP2C cDNAs replaced with those of bovine 17α hydroxylase (MALLLAVF) to optimize bacterial expression.
Proper citation: RRID:Addgene_69606 Copy
Species: Homo sapiens
Genetic Insert: UBA52
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3626; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26235645
Comments: Human and mouse UBA52 are identical at protein level.
Proper citation: RRID:Addgene_69560 Copy
Species: Homo sapiens
Genetic Insert: CYP2C8
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCW; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Modification of CYP2C8 for bacterial expression as per Barnes et al. PNAS 88:5597, 1991 [PMID: 1829523]: First 8 amino acids of CYP2C cDNAs replaced with those of bovine 17α hydroxylase (MALLLAVF) to optimize bacterial expression. An XbaI site was placed after the stop codon The insert was ligated into NdeI and XBbaI cut pCW ori+.
Proper citation: RRID:Addgene_69604 Copy
Species: Homo sapiens
Genetic Insert: CENP-R
Vector Backbone Description: Vector Backbone:pJAG98 (pBABEblast); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69738 Copy
Species: Homo sapiens
Genetic Insert: Bod1
Vector Backbone Description: Vector Backbone:pJAG98 (pBABEblast); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69737 Copy
Species: Homo sapiens
Genetic Insert: PELP1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_69736 Copy
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