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Species: Homo sapiens
Genetic Insert: ErbB4
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:3500; Vector Backbone:pM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12807903
Proper citation: RRID:Addgene_17800 Copy
Species: Homo sapiens
Genetic Insert: ErbB4
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:3500; Vector Backbone:pM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12807903
Proper citation: RRID:Addgene_17802 Copy
Species: Synthetic
Genetic Insert: PEmax
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Template for in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34653350
Comments: Addgene's NGS results identified a one base pair mismatch in the RT region of the plasmid relative to the depositor's reference sequence. The mismatch does not impact the amino acid sequence and is not predicted to impact plasmid function.
Proper citation: RRID:Addgene_178113 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178119 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control pegRNA vector derived from ATP1A1_G6_T804N_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA) to co-target ATP1A1 (T804N) and MTOR to perform coselection for MTOR rapamycin resistance mutation F2108L. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178109 Copy
Genetic Insert: ATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control pegRNA vector derived from ATP1A1_G4_Q118R_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA) to co-target ATP1A1 (Q118R) and MTOR to perform coselection for MTOR rapamycin resistance mutation F2108L. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178102 Copy
Genetic Insert: ATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control pegRNA vector derived from ATP1A1_G6_T804N_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA) to co-target ATP1A1 (T804N) and MTOR to perform coselection for MTOR hyperactivating mutation I2017T. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178108 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G8 + MTOR nick intron-45 sgRNAs
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control PE3 nick sgRNAs vector derived from ATP1A1_G8_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 (T804N) and MTOR to perform coselection for MTOR rapamycin resistance mutation F2108L. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178106 Copy
Species: Homo sapiens
Genetic Insert: WLS
Vector Backbone Description: Backbone Marker:Addgene (#51406); Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34587386
Proper citation: RRID:Addgene_178064 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178136 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G3 + EBFP nick sgRNAs
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 (Q118R) and EBFP to perform coselection for EBFP to EGFP conversion. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178094 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178125 Copy
Species: A. victoria
Genetic Insert: GFPuv
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16333863
Comments: Provided in strain JSB, a slightly modified version of E. coli strain BL21 with genotype: BL21(DE3)/D(sbm-ygfDGHI).
Proper citation: RRID:Addgene_17813 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178141 Copy
Vector Backbone Description: Backbone Size:5135; Vector Backbone:pPro18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16269719
Comments: The PCR product containing prpR and PprpB was digested with ClaI and NheI and ligated to the large fragments of pBAD18 resulting from digestion with the same enzymes, creating pPro18.
Please refer to the Addgene verified sequence for the correct orientation of the MCS.
Proper citation: RRID:Addgene_17806 Copy
Vector Backbone Description: Backbone Size:5445; Vector Backbone:pPro30; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16269719
Comments: After deletion of the NheI site in the front of pACYC origin region of pBAD30 by partial digestion with NheI, T4 DNA polymerase treatment, and self-ligation, the ClaI-NheI prpR-PprpB region from pPro18 was ligated to the large fragments of pBAD30 resulting from digestion with the same enzymes, creating pPro30.
Proper citation: RRID:Addgene_17809 Copy
Species: Synthetic
Genetic Insert: Lrig1 shRNAmir
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, RNAi, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.
Proper citation: RRID:Addgene_178048 Copy
Species: Synthetic
Genetic Insert: Renilla_713 shRNAmir
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, RNAi, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.
Proper citation: RRID:Addgene_178046 Copy
Species: Synthetic
Genetic Insert: Pten_1523 shRNAmir
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, RNAi, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.
Proper citation: RRID:Addgene_178044 Copy
Species: Synthetic
Genetic Insert: Luciferase_1309 shRNAmir
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, RNAi, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.
Proper citation: RRID:Addgene_178045 Copy
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