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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pUT1754-HQ858876
 
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RRID:Addgene_27958 bHLH transcription factor encoded by Os03g0759700 LOC_Os03g55220 Oryza sativa (rice) Kanamycin PMID:18987217 This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector. Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert) Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:13:08 0
pUT1750-HQ858873
 
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RRID:Addgene_27955 WRKY transcription factor encoded by Os01g0714800 LOC_Os01g51690 Oryza sativa (rice) Kanamycin PMID:18987217 This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector. Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert) Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:13:08 0
pUT1684-HQ858871
 
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RRID:Addgene_27953 bHLH transcription factor encoded by Os03g0741100 LOC_Os03g53020 Oryza sativa (rice) Kanamycin PMID:18987217 This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector. Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert) Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 0
pUT1678-HQ858869
 
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RRID:Addgene_27951 bHLH transcription factor encoded by Os08g0524800 LOC_Os08g41320 Oryza sativa (rice) Kanamycin PMID:18987217 This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector. Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert) Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 0
monomer-HD
 
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1+ mentions
RRID:Addgene_27968 TAL-monomer-HD Xanthomonas Kanamycin PMID:21248753 Backbone Size:2672; Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 1
monomer-NI
 
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1+ mentions
RRID:Addgene_27966 TAL-monomer-NI Xanthomonas Kanamycin PMID:21248753 Backbone Size:2672; Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 1
monomer-NG
 
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1+ mentions
RRID:Addgene_27967 TAL-monomer-NG Xanthomonas Kanamycin PMID:21248753 Backbone Size:2672; Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:08 1
monomer-NN
 
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1+ mentions
RRID:Addgene_27965 TAL-monomer-NN Xanthomonas Kanamycin PMID:21248753 Backbone Size:2672; Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 1
pUT1858-HQ858878
 
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RRID:Addgene_27960 bHLH transcription factor encoded by Os01g0928000 LOC_Os01g70310 Oryza sativa (rice) Kanamycin PMID:18987217 This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector. Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert) Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 0
NFATc1_R (OZ544)
 
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RRID:Addgene_28069 Zinc finger array targeting NFATc1 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene NFATc1(see below). Please note that this plasmid does NOT contain the NFATc1 sequence. Users must order the complementary plasmid NFATc1_L (OZ543) [Addgene plasmid 28068] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGGA-3' in a bacterial two hybrid assay, and resulted in 7.97 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
Encephalopsin (Opn3)_L (OZ541)
 
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RRID:Addgene_28066 Zinc finger array targeting Encephalopsin (Opn3) Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Encephalopsin (Opn3)(see below). Please note that this plasmid does NOT contain the Encephalopsin (Opn3) sequence. Users must order the complementary plasmid Encephalopsin (Opn3)_R (OZ542) [Addgene plasmid 28067] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TGGGATGGA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
Atg7_R (OZ558)
 
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RRID:Addgene_28083 Zinc finger array targeting Atg7 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Atg7 (see below). Please note that this plasmid does NOT contain the Atg7 sequence. Users must order the complementary plasmid Atg7_L (OZ557) [Addgene plasmid 28082] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GTGGTGGCT-3' in a bacterial two hybrid assay, and resulted in 6.91 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
cdkn1a(p21)_L (OZ555)
 
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RRID:Addgene_28080 Zinc finger array targeting cdkn1a(p21) Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene cdkn1a(p21) (see below). Please note that this plasmid does NOT contain the cdkn1a(p21) sequence. Users must order the complementary plasmid cdkn1a(p21)_R (OZ556) [Addgene plasmid 28081] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGTGAACG-3' in a bacterial two hybrid assay, and resulted in 14.32 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
REST corepressor 2_R (OZ554)
 
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RRID:Addgene_28079 Zinc finger array targeting REST corepressor 2 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene REST corepressor 2 (see below). Please note that this plasmid does NOT contain the REST corepressor 2 sequence. Users must order the complementary plasmid REST corepressor 2_L (OZ553) [Addgene plasmid 28078] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TGCGATGGA-3' in a bacterial two hybrid assay, and resulted in 6.66 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 0
GUSB_R (OZ552)
 
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RRID:Addgene_28077 Zinc finger array targeting GUSB Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene GUSB (see below). Please note that this plasmid does NOT contain the GUSB sequence. Users must order the complementary plasmid GUSB_L (OZ551) [Addgene plasmid 28076] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGCGGAC-3' in a bacterial two hybrid assay, and resulted in 11.33 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
REST corepressor 2_L (OZ553)
 
Resource Report
Resource Website
RRID:Addgene_28078 Zinc finger array targeting REST corepressor 2 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene REST corepressor 2 (see below). Please note that this plasmid does NOT contain the REST corepressor 2 sequence. Users must order the complementary plasmid REST corepressor 2_R (OZ554) [Addgene plasmid 28079] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GATGGTGAG-3' in a bacterial two hybrid assay, and resulted in 10.65 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
histamine receptor H3 (hrh3)_R (OZ550)
 
Resource Report
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RRID:Addgene_28075 Zinc finger array targeting histamine receptor H3 (hrh3) Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene histamine receptor H3 (hrh3) (see below). Please note that this plasmid does NOT contain the histamine receptor H3 (hrh3) sequence. Users must order the complementary plasmid histamine receptor H3 (hrh3)_L (OZ549) [Addgene plasmid 28074] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGACTGC-3' in a bacterial two hybrid assay, and resulted in 12.10 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
pSL7 SMR1791
 
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RRID:Addgene_28030 MutS, MutL E. coli Kanamycin PMID:9308969 pSL7 contains a ClaI–BglII fragment of pSL6 (carrying mutS), a BamHI–HindIII fragment of pKC31 (conferring Kanamycin resistance), and a HindIII–PstI fragment of pAL51 (carrying mutL) Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
pSL5 SMR1713
 
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RRID:Addgene_28028 MutL E. coli Kanamycin PMID:9308969 pSL5 contains a PstI–HindIII fragment of pAL51 carrying E. coli mutL and a KanR-conferring HindIII–BamHI fragment of pKC31 replacing digest sites PstI and BamHI of pBR322 Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 0
pTGV-Express-Kan SMR5917
 
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RRID:Addgene_28038 Kanamycin PMID:11483365 pTGV-Express contains PBAD and also includes the Shine-Dalgarno prokaryotic translation-initiation sequence for expression of heterologous genes in E. coli. Backbone Size:7990; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0

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