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Species: Homo sapiens
Genetic Insert: HURP
Vector Backbone Description: Backbone Marker:Arshad Desai (Addgene plasmid# 44432); Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69748 Copy
Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Vector Backbone:pIC194; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_69747 Copy
Species: Homo sapiens
Genetic Insert: Centrin 3
Vector Backbone Description: Backbone Marker:Arshad Desai (Addgene plasmid# 44432); Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69746 Copy
Species: Homo sapiens
Genetic Insert: Centrin 1
Vector Backbone Description: Backbone Marker:Arshad Desai (Addgene plasmid# 44432); Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69744 Copy
Species: Homo sapiens
Genetic Insert: DSN1
Vector Backbone Description: Backbone Marker:Arshad Desai (Addgene plasmid# 44432); Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23530067
Proper citation: RRID:Addgene_69754 Copy
Species: Homo sapiens
Genetic Insert: spc24
Vector Backbone Description: Backbone Marker:Arshad Desai (Addgene plasmid# 44432); Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69752 Copy
Species: Homo sapiens
Genetic Insert: CENP-T aa 1-375
Vector Backbone Description: Vector Backbone:pJAG98 (pBABEblast); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69761 Copy
Species: Homo sapiens
Genetic Insert: 77,406 unique sgRNAs covering 20,121 genes in the human genome
Vector Backbone Description: Backbone Marker:Stephan Kissler lab; Backbone Size:8831; Vector Backbone:pLB (Addgene Plasmid #11619); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26190106
Comments: This sgRNA library was synthesized as an oligo pool, with 77,406 unique sgRNAs covering 20,121 genes in the human genome (see attached supplemental file for genes and target sequences).
To ensure complete gene disruption, the target sites were chosen in a region close to the translation initiation site, and most genes were covered four times. It is noteworthy that the sgRNAs start with either A or G, because our previous study showed that the preferred transcription initiation nucleotide for the U6 promoter can be either A or G (Ma et al., 2014).
The sgRNA oligo pool was PCR amplified and cloned into the lentiviral vector, pLB. To test its quality, we PCR amplified the library and analyzed the amplicons with deep sequencing. The results showed that at least 90% of the sgRNAs were retained in the plasmid library (see attached supplemental file for sequencing read counts).
For more information about library use and screening, please see the associated publication (Ma et al. Cell Reports 2015, PMID: 26190106)
This library will be delivered in a microcentrifuge tube on blue ice. You will receive ~20µL of DNA at a concentration of 50ng/µL. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Before ordering, please review the steps for using pooled lentiviral CRISPR libraries at https://www.addgene.org/crispr/lentiviral/library/reference/
Proper citation: RRID:Addgene_69763 Copy
Species: Homo sapiens
Genetic Insert: PCGF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_69770 Copy
Species: Homo sapiens
Genetic Insert: Apoptosis signal-regulating kinase 3
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_69728 Copy
Species: Homo sapiens
Genetic Insert: ZKSCAN1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Proper citation: RRID:Addgene_69901 Copy
Species: Homo sapiens
Genetic Insert: ciRS7
Vector Backbone Description: Backbone Size:6926; Vector Backbone:pcDNA3.1(+) Laccase2 MCS Exon Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Proper citation: RRID:Addgene_69900 Copy
Species: Homo sapiens
Genetic Insert: GIV/Girdin
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET-28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21209316
Proper citation: RRID:Addgene_69864 Copy
Species: Homo sapiens
Genetic Insert: ZKSCAN1
Vector Backbone Description: Backbone Size:8622; Vector Backbone:Hy_pMT Laccase2 MCS Exon Vector; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Comments: Please note that there is an additional 84 amino acids that will be expressed on the C-terminus of the ZKSCAN1 (aa1-137) insert in this plasmid.
Proper citation: RRID:Addgene_69886 Copy
Species: Homo sapiens
Genetic Insert: CDC42 effector protein
Vector Backbone Description: Backbone Size:1254; Vector Backbone:pcDNA 3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26456332
Proper citation: RRID:Addgene_69819 Copy
Species: Homo sapiens
Genetic Insert: SH3 and PX domain-containing protein 2A
Vector Backbone Description: Backbone Size:2898; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26456332
Proper citation: RRID:Addgene_69816 Copy
Species: Homo sapiens
Genetic Insert: Sorting nexin-17
Vector Backbone Description: Backbone Size:2898; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26456332
Proper citation: RRID:Addgene_69812 Copy
Species: Homo sapiens
Genetic Insert: ZKSCAN1
Vector Backbone Description: Backbone Size:6926; Vector Backbone:pcDNA3.1(+) Laccase2 MCS Exon Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Proper citation: RRID:Addgene_69896 Copy
Species: Homo sapiens
Genetic Insert: ZKSCAN1
Vector Backbone Description: Backbone Size:6926; Vector Backbone:pcDNA3.1(+) Laccase2 MCS Exon Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Proper citation: RRID:Addgene_69894 Copy
Species: Homo sapiens
Genetic Insert: Mitochondrial fission regulator 1-like
Vector Backbone Description: Backbone Size:5394; Vector Backbone:pcDNA 3.2/V5-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26456332
Comments: vector is Gateway and cDNA is from Orfeome library (Open Biosystems)
Proper citation: RRID:Addgene_69822 Copy
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