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Species: Synthetic
Genetic Insert: 2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and turboGFP expression vector for plants. Introns avoid expression in Agrobacteria. Increased virulence in Agrobacteria through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123190 Copy
Species: Synthetic
Genetic Insert: luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, Luciferase, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, Renilla luciferase with an intron for transient and stable expression in plants. Codon optimized for plants.
Proper citation: RRID:Addgene_123181 Copy
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLV-CMV-y/hNubI-tripleFLAG-linker-Gateway-PuroR; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123221 Copy
Species: Agrobacterium tumefaciens
Genetic Insert: VirGN54D from pAD1289 (a gift from A. Das)
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:12773; Vector Backbone:pAGM4723; Vector Types:Plant Expression, Level 2 acceptor vector for expression in plants ; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo; Weber et al., 2011) level 2 acceptor vector with Mutation VirGN54D for increased virulence. Increased virulence in Agrobacteria for a high-efficiency gene transfer (increased with and without acetosyringone, compared to original vector control).
Proper citation: RRID:Addgene_123187 Copy
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLV-CMV-y/hNubI-tripleFLAG-linker-Gateway-PuroR; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123223 Copy
Species: P1 coliphage
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:5100; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31028261
Proper citation: RRID:Addgene_123133 Copy
Species: Mycobacterium smegmatis
Genetic Insert: Bxb1 integrase
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31028261
Proper citation: RRID:Addgene_123132 Copy
Species: Mus musculus
Genetic Insert: ATG5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3941; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16645637
Comments: for mCherry marker protein
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA,
Tsien RY. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82
Proper citation: RRID:Addgene_13095 Copy
Species: Mus musculus
Genetic Insert: ATG5-K130R
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3941; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16645637
Comments: for mCherry marker protein
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82
Proper citation: RRID:Addgene_13096 Copy
Species: Homo sapiens
Genetic Insert: KIF1C-GFP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344256
Proper citation: RRID:Addgene_130977 Copy
Species: Homo sapiens
Genetic Insert: KIF1C-GFP
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pFastBacM13; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31217419
Proper citation: RRID:Addgene_130975 Copy
Species: Synthetic
Genetic Insert: FnCas9
Vector Backbone Description: Backbone Size:5023; Vector Backbone:PX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31570623
Proper citation: RRID:Addgene_130969 Copy
Species: Synthetic
Genetic Insert: 3xHA-NLS-SpCas9
Vector Backbone Description: Vector Backbone:PX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31570623
Proper citation: RRID:Addgene_130968 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31320556
Proper citation: RRID:Addgene_131002 Copy
Species: Tiarina fusus
Genetic Insert: ChRmine
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31320556
Proper citation: RRID:Addgene_130998 Copy
Species: Tiarina fusus
Genetic Insert: ChRmine
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31320556
Proper citation: RRID:Addgene_130994 Copy
Species: Tiarina fusus
Genetic Insert: ChRmine
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31320556
Proper citation: RRID:Addgene_130995 Copy
Species: Tiarina fusus
Genetic Insert: ChRmine
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31320556
Proper citation: RRID:Addgene_130991 Copy
Species: Synthetic
Genetic Insert: NLS-HA-2xMCP-mCherry-BirA*
Vector Backbone Description: Backbone Size:6248; Vector Backbone:pHAGE-UBC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Variant of phage - ubc - nls - 2xmcp - egfp - BirA* (Addgene plasmid 131132) with two copies of MCP protein and mCherry instead of eGFP. Consequently, binding of two MCPs per MS2 RNA loop results in twice the number of BirA* proteins than in previous cassettes
Proper citation: RRID:Addgene_131136 Copy
Species: Synthetic
Genetic Insert: NLS-HA-2xMCP-EGFP-BirA*
Vector Backbone Description: Backbone Size:6248; Vector Backbone:pHAGE-UBC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31189591
Proper citation: RRID:Addgene_131132 Copy
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