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Species: Homo sapiens
Genetic Insert: PMCA2w/b
Vector Backbone Description: Backbone Marker:Penniston Lab; Backbone Size:5145; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12624087
Comments: w/b splice variant of human PMCA2. PMCA2 start: 1090; stop: 4821, w-splice: 1997-2131; b-splice: 4510-4821
Proper citation: RRID:Addgene_47583 Copy
Species: Mus musculus
Genetic Insert: Notch3 Intracellular domain
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression, in vitro transcription and translation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8951795
Proper citation: RRID:Addgene_47618 Copy
Species: Danio rerio
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47619 Copy
Species: Homo sapiens
Genetic Insert: MKK4-EE
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47577 Copy
Vector Backbone Description: Backbone Size:10000; Vector Backbone:pI3 and pMTL23; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10966401
Proper citation: RRID:Addgene_47698 Copy
Species: Homo sapiens
Genetic Insert: MyoX
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFPN2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47611 Copy
Species: Homo sapiens
Genetic Insert: Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6645; Vector Backbone:pmalC4X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21612213
Comments: The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
Proper citation: RRID:Addgene_47699 Copy
Species: Mus musculus
Genetic Insert: MEK1-DD
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47576 Copy
Species: Homo sapiens
Genetic Insert: MKK6-EE
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47579 Copy
Species: Mus musculus
Genetic Insert: Notch1 intracellular domain
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4t-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10433920
Comments: Construct also called GST-1111.
Proper citation: RRID:Addgene_47612 Copy
Species: Homo sapiens
Genetic Insert: JNK1a1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5053; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47574 Copy
Species: Homo sapiens
Genetic Insert: MyoX
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2001; Backbone Size:4700; Vector Backbone:EGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17130134
Comments: hMyoX contains a Q680R variant compared to the NCBI reference [AAF37875.1]. The plasmid should function as described in the associated publication.
Proper citation: RRID:Addgene_47608 Copy
Species: Homo sapiens
Genetic Insert: CALML3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11278607
Proper citation: RRID:Addgene_47605 Copy
Species: Synthetic
Genetic Insert: Codon-optimised P12p
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47726 Copy
Species: Synthetic
Genetic Insert: Codon-optimised P38
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47727 Copy
Species: Homo sapiens
Genetic Insert: CALM3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pGEMEX-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9828009
Comments: We observed in preliminary experiments that IPTG added to the growth media did not induce substantial additional CLP expression. In addition, we found that the addition of glucose to the growth medium significantly inhibited CLP expression from this vector. An NdeI site at position 3259 has been abolished.
Proper citation: RRID:Addgene_47600 Copy
Species: Homo sapiens
Genetic Insert: CaM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11278607
Proper citation: RRID:Addgene_47603 Copy
Species: Homo sapiens
Genetic Insert: CALML3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47604 Copy
Species: Synthetic
Genetic Insert: Codon-optimised P12
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47725 Copy
Species: Homo sapiens
Genetic Insert: CaM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47602 Copy
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