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Species: Synthetic
Genetic Insert: TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
Vector Backbone Description: Backbone Size:6480; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32483365
Comments: Please visit https://www.biorxiv.org/content/10.1101/729269v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_131124 Copy
Vector Backbone Description: Backbone Marker:Sikorski and Hieter, 1994; Vector Backbone:pRS314 (exchange of TRP1 against kanMX); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21938623
Proper citation: RRID:Addgene_131168 Copy
Species: Kaposi's Sarcoma Herpesvirus
Genetic Insert: KSHV Lytic Origin of replication
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6075; Vector Backbone:pGL4.16; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31095645
Proper citation: RRID:Addgene_131038 Copy
Genetic Insert: humanised S. pyogenes Cas9 nuclease
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 42230); Backbone Size:8506; Vector Backbone:pX330-U6-Chimeric_BB-Cbh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33279577
Proper citation: RRID:Addgene_131257 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin carboxyl-terminal hydrolase 7
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:5435; Vector Backbone:pcDNA3.1+/N-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33279577
Proper citation: RRID:Addgene_131243 Copy
Species: Homo sapiens
Genetic Insert: Opto-casp9 (AtCRY2-PHR-casp9)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5318; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31150735
Proper citation: RRID:Addgene_131221 Copy
Species: Homo sapiens
Genetic Insert: NOD2
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33942347
Comments: For cloning Nod2, variant 1 was used. Nod1wt was cut out and Nod2 without stop codon was integrated. To ensure that EGFP and Nod2 are in-frame, two additional bases were added at reverse primer (primer without stop codon of Nod2).
Insert has three silent mutations:
Codon 178: TCG instead of TCC --> Serine
Codon 587: CCT instead of CCC --> Proline
Codon 792: CGG instead of CGT --> Arginine
Proper citation: RRID:Addgene_131207 Copy
Species: Homo sapiens
Genetic Insert: hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
Vector Backbone Description: Backbone Marker:pSQT817 (Addgene #53373); Backbone Size:4843; Vector Backbone:CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31477922
Proper citation: RRID:Addgene_131315 Copy
Species: Homo sapiens
Genetic Insert: hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
Vector Backbone Description: Backbone Marker:pSQT817 (Addgene #53373); Backbone Size:4843; Vector Backbone:CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31477922
Proper citation: RRID:Addgene_131314 Copy
Species: E.coli, Streptococcus pyogenes
Genetic Insert: bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31477922
Proper citation: RRID:Addgene_131312 Copy
Species: Homo sapiens
Genetic Insert: ORFeus
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32042151
Proper citation: RRID:Addgene_131386 Copy
Species: Homo sapiens
Genetic Insert: ORFeus-Hs
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32042151
Proper citation: RRID:Addgene_131380 Copy
Species: Mus musculus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9254; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_131484 Copy
Species: Mus musculus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9253; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_131479 Copy
Species: Mus musculus
Genetic Insert: MyD88-CpLxIS
Vector Backbone Description: Vector Backbone:pMSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30853218
Proper citation: RRID:Addgene_131348 Copy
Species: Synthetic
Genetic Insert: Streptomyces codon optimized spCas9n (D10A)
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548381
Proper citation: RRID:Addgene_131464 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y819W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1314 Copy
Species: Homo sapiens
Genetic Insert: L1RP GFP-AI
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32042151
Proper citation: RRID:Addgene_131392 Copy
Species: Homo sapiens
Genetic Insert: ORFeus
Vector Backbone Description: Vector Backbone:pSBtet-RN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32042151
Proper citation: RRID:Addgene_131390 Copy
Species: Synthetic
Genetic Insert: RT-1306
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31548705
Proper citation: RRID:Addgene_131521 Copy
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