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Species: Other
Genetic Insert: Cas9-BFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34697476
Comments: See bioRxiv article for preprint info - https://www.biorxiv.org/content/10.1101/775080v2
Proper citation: RRID:Addgene_174087 Copy
Species: Other
Genetic Insert: dCasRx-METTL3
Vector Backbone Description: Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34181729
Proper citation: RRID:Addgene_174218 Copy
Species: Other
Genetic Insert: Sirius with mitchondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174184 Copy
Species: Other
Genetic Insert: protein AG - hia5
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35396487
Comments: Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye
Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383
Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646
This plasmid has been observed to occur in our stock as a plasmid dimer. Dimerization often does not impact plasmid function, but may reduce transformation and transfection efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid in a recombinase-deficient strain.
Proper citation: RRID:Addgene_174373 Copy
Species: Other
Genetic Insert: PylS_1R26 - CBZK
Vector Backbone Description: Backbone Size:3068; Vector Backbone:pMB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34083482
Proper citation: RRID:Addgene_174516 Copy
Species: Other
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:3896; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:34083482
Proper citation: RRID:Addgene_174518 Copy
Species: Other
Genetic Insert: SECFP with actin-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174476 Copy
Species: Other
Genetic Insert: dCasRx-ALKBH5
Vector Backbone Description: Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34181729
Proper citation: RRID:Addgene_175582 Copy
Species: Other
Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:lenti-BSD; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34181729
Proper citation: RRID:Addgene_175583 Copy
Species: Other
Genetic Insert: Nluc(W12stop)-2xC/D box
Vector Backbone Description: Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477620v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_180505 Copy
Species: Other
Genetic Insert: MAC-C SARS-CoV-2 Omicron spike protein
Vector Backbone Description: Vector Backbone:MAC-tag-C (#108077); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180778 Copy
Species: Other
Genetic Insert: KDRT
Vector Backbone Description: Vector Backbone:pENTR; Vector Types:pENTR plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182165 Copy
Species: Other
Genetic Insert: mTurquoise
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182181 Copy
Species: Other
Genetic Insert: mTurquoise
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182180 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182183 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182185 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182199 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182196 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182167 Copy
Species: Other
Genetic Insert: mTurquoise
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182169 Copy
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