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Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4004; Vector Backbone:pEGPF-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18267093
Proper citation: RRID:Addgene_23007 Copy
Species: Caenorhabditis elegans
Genetic Insert: SnAvi
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9674; Vector Backbone:EGFP-N1; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20047968
Comments: this plasmid encodes the new described SnAvi tag and additionally the unc-119 rescue sequence from C. elegans (bp4-5705)
Proper citation: RRID:Addgene_23223 Copy
Species: Helicobacter pylori
Genetic Insert: TNF-α-inducing protein
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19596016
Proper citation: RRID:Addgene_23159 Copy
Species: Helicobacter pylori
Genetic Insert: TNF-α-inducing protein
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19596016
Proper citation: RRID:Addgene_23157 Copy
Species: Helicobacter pylori
Genetic Insert: TNF-α-inducing protein
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19596016
Proper citation: RRID:Addgene_23158 Copy
Species: Mus musculus
Genetic Insert: pCMV Tag2b Bim S
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18498746
Proper citation: RRID:Addgene_23283 Copy
Species: Homo sapiens
Genetic Insert: PLK3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18519666
Proper citation: RRID:Addgene_23268 Copy
Species: Homo sapiens
Genetic Insert: Set9
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11850410
Proper citation: RRID:Addgene_24083 Copy
Species: Homo sapiens
Genetic Insert: PTPRN
Vector Backbone Description: Backbone Size:5051; Vector Backbone:pFC14K; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_24057 Copy
Species: Homo sapiens
Genetic Insert: CLASP2 (340-1084) 9xS/A
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.
Proper citation: RRID:Addgene_24384 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24402 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24403 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pENTR2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21375737
Comments: Contains Gateway L1L3 sites. PacI and Sbf1 can be used to insert promoter of interest to drive tTA expression. Contains insulator sequence.
Plasmid was constructed as follows: The pEntr2B entry vector was digested with PstI and XhoI to remove the attL2 site. Oligonucleotides containing SalI-XhoI-Bsu36I-NdeI-PacI-EcoRI-NotI flanking the attL3 sequence on the 5' side and PstI on the 3' side were ligated in to create an intermediate plasmid carrying attL1 and attL3 sites (from
pDest R4-R3. A LoxP sequence was introduced at the XhoI site, and the 500-bp chicken β-globin (CBG) HS4 insulator sequence was introduced at the Bsu36I/NdeI sites. The tTA and pA sequences were
subsequently cloned into the EcoRI/NotI sites by PCR cloning from pUHG15-1 to generate pEntL1L3 tTA-
2
Proper citation: RRID:Addgene_24414 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pENTR2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21375737
Comments: Contains Gateway R3L2 sites. Full length (7 repeat) TetO. SbfI, FseI, AvrII, and SalI sites can be used to insert gene of interest, driven by TetO. Contains insulator sequence
Plasmid was constructed as follows: The pEntr2B entry vector was digested with AflII and XhoI to remove the attL1 and ccdb genes. Oligos
containing the attR3 site flanked by AflII on the 5' end and a polylinker (NotI-PacI-Bsu36I-NdeI-XhoI) on the 3' end were ligated to generate the empty pEntR3L2-MCS intermediate (Addgene plasmid # 26799). Using PCR cloning, the TetO-beta globin
intron sequence from pTetO (pUHD10.3) was cloned into the NotI/PacI sites in the reverse orientation,
and a 3' SbfI site was introduced. The pA sequence from pDest27 was then cloned into the NotI/SbfI sites, again in reverse orientation. A LoxP site was introduced into the NdeI/XhoI sites, allowing full
excision of the targeted construct when used together with the LoxP site in the pEntl1L3 vector. The CBG core
insulator was then cloned into the Bsu36I/NdeI sites to generate the starting entry vector pEntR3L2 TetO(fl)-2.
Proper citation: RRID:Addgene_24416 Copy
Species: Homo sapiens
Genetic Insert: CLASP2 (340-1362)
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.
Proper citation: RRID:Addgene_24372 Copy
Species: Homo sapiens
Genetic Insert: GSK3beta(S9A)
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: Addgene's sequence results have identified a duplicated mRFP1 and GSK S9A in this plasmid. The depositor has confirmed that this duplication should not impact plasmid functionality.
Proper citation: RRID:Addgene_24371 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Proper citation: RRID:Addgene_24327 Copy
Species: Homo sapiens
Genetic Insert: transcription factor Sp3
Vector Backbone Description: Backbone Marker:Guntram Suske, Addgene plasmid 24544; Backbone Size:4000; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15247228
Comments: Two nucleotide mismatches with author sequence detected (one adjacent to the EcoRI cloning site and the second in the middle of the 5´-UTR). They do not affect the coding sequence of Sp3.
Proper citation: RRID:Addgene_24541 Copy
Genetic Insert: pN3-Control
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pN3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15247228
Comments: vector control for pN3-Sp1FL and pN3-Sp3FL vectors
Proper citation: RRID:Addgene_24544 Copy
Species: Homo sapiens
Genetic Insert: Sp1FL
Vector Backbone Description: Backbone Marker:Guntram Suske, Addgene plasmid 24544; Backbone Size:4000; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: pN3-Control vector also available from Addgene
2 nucleotide mismatches with author sequence detected in the most 3´-UTR. They do not affect the coding sequence of Sp1.
Proper citation: RRID:Addgene_24543 Copy
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