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Species: Synthetic
Genetic Insert: PdCO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3785; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38811857
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.01.547328v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_198508 Copy
Species: Synthetic
Genetic Insert: PdCO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3785; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38811857
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.01.547328v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_198514 Copy
Species: Synthetic
Genetic Insert: PdCO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3899; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38811857
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.01.547328v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_198513 Copy
Species: Synthetic
Genetic Insert: PdCO
Vector Backbone Description: Backbone Size:3899; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38811857
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.01.547328v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_198510 Copy
Species: Synthetic
Genetic Insert: PdCO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5616; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38811857
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.01.547328v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_198511 Copy
Species: Synthetic
Genetic Insert: NPTII
Vector Backbone Description: Backbone Marker:Yiping Qi; Vector Backbone:pYPQ210; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:37070560
Comments: A 185T mutation was found in mCherry.
Proper citation: RRID:Addgene_199721 Copy
Species: Synthetic
Genetic Insert: stChrimsonR, PdCO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3913; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38811857
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.01.547328v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_202199 Copy
Species: Synthetic
Genetic Insert: stChrimsonR, PdCO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3762; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38811857
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.01.547328v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_202198 Copy
Species: Synthetic
Genetic Insert: Cre-dependent, membrane-localized Optopatch
Vector Backbone Description: Backbone Size:5305; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39905023
Comments: Please visit https://doi.org/10.1101/2023.06.02.543490 for bioRxiv preprint.
Proper citation: RRID:Addgene_203229 Copy
Species: Synthetic
Genetic Insert: FKBP-rapamycin binding (FRB) dimerization domain
Vector Backbone Description: Vector Backbone:pQCXIZ BFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36735782
Proper citation: RRID:Addgene_202426 Copy
Species: Synthetic
Genetic Insert: TcCas12b
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31016029
Proper citation: RRID:Addgene_188277 Copy
Species: Synthetic
Genetic Insert: PlmCasX
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35693153
Proper citation: RRID:Addgene_188273 Copy
Species: Synthetic
Genetic Insert: PlmCasX(T26R/K610R/K808R)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35693153
Proper citation: RRID:Addgene_188274 Copy
Species: Synthetic
Genetic Insert: Cas12i2
Vector Backbone Description: Vector Backbone:BPK2014; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35693153
Proper citation: RRID:Addgene_188275 Copy
Species: Synthetic
Genetic Insert: Cre-PhoCI-ERT2-ERT2
Vector Backbone Description: Backbone Marker:Vlatka Zoldos; Backbone Size:8020; Vector Backbone:#71666 pdCas9-DNMT3A-EGFP; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36307419
Comments: Cloning was performed using a third generation lentiviral plasmid as a backbone (pdCas9-DNMT3A-EGFP (Vlatka Zoldoš, #71666)) digested with NheI-EcoRI and ligated to the following DNA fragments: TREGV 3rd generation promoter (amplified by PCR using TRE-KRAB-dCas9-IRES-GFP (Eric Lander, #85556) as a template and digested with NheI-XmaI), PhoCl-ERT2-Cre-ERT2-PhoCl sequence (pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 (Robert Campbell, #87694)) digested with XmaI-NotI, and NotI-EcoRI adapter. From construct PhoCl-ERT2-Cre-ERT2-PhoCl, the sequence Cre-PhoCl-ERT2 was amplified. Cre sequence from the original construct lacked the canonical first 16 aminoacids (MANLLTVHQNLPALPV), so we restored them. The synthesized fragment was ligated into the pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 vector upon PacI and EcoRI digestion. Using this as a template, we amplified Cre-PhoCl-ERT2 sequence to generate a new fragment flanked by PacI and AscI restriction sites. An additional ERT2 domain was synthetized by PCR with AscI and EcoRI ends and one 5′-GSGSGGG flexible linker. Upon digestion, the two fragments were ligated into the pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 vector upon PacI and EcoRI digestion. Finally, Cre-PhoCl-ERT2(x2) fragment was jumped into the PiggyBac XLone vector system25. To this end, XLone plasmid backbone was PCR and Cre-PhoCl-ERT2(x2) cassette ligated upon NheI-NotI digestion.
Proper citation: RRID:Addgene_198752 Copy
Species: Synthetic
Genetic Insert: Adeno_NES-EGFP-4PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG050/P303
Proper citation: RRID:Addgene_201355 Copy
Species: Synthetic
Genetic Insert: MAPK_NES-EGFP
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG066/P526
Proper citation: RRID:Addgene_201366 Copy
Species: Synthetic
Genetic Insert: mScaret-I
Vector Backbone Description: Vector Backbone:pPOT; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40169015
Comments: Please visit https://doi.org/10.1101/2023.04.21.537815 for bioRxiv preprint.
Proper citation: RRID:Addgene_201091 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40-EGFP-3PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG071/P531
Proper citation: RRID:Addgene_201370 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40A4-EGFP-1PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG058/P311
Proper citation: RRID:Addgene_201358 Copy
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