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Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pZ F3 CAGGS GPHTK F (AddGene plasmid 112666); Vector Types:Mammalian Expression, AAV, Donor plasmid for recombinase-mediated cassette exchange; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26455413
Proper citation: RRID:Addgene_115518 Copy
Species: Other
Genetic Insert: ccdB-sgRNA scaffold
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EF1a-puromycin; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_115519 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pTcTurboID-GW; Vector Types:Trypanosomatid expression; Bacterial Resistance:Ampicillin
Comments: If you want to perform traditional cloning (instead of Gateway recombiantion), it can be achieved by replacing the GFP cassette from pTcTurboID-GW_GFP with the desired CDS using BamHI and EcoRV restriction enzymes. Also, any CDS can be cloned in frame to GFP using only EcoRV.
Proper citation: RRID:Addgene_234715 Copy
Species: Other
Genetic Insert: Lck-mScarlet3
Vector Backbone Description: Vector Backbone:Addgene plasmid #107702; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40576868
Proper citation: RRID:Addgene_234540 Copy
Species: Other
Genetic Insert: Lck-mTagBFP2
Vector Backbone Description: Vector Backbone:Addgene plasmid #107702; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40576868
Proper citation: RRID:Addgene_234537 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.; Vector Backbone:pJRT.Agro.CLCrVA.008; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40394900
Proper citation: RRID:Addgene_234917 Copy
Species: Other
Genetic Insert: guide targeting fwYellow
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR, Cell-free system using bacterial extract; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39095367
Proper citation: RRID:Addgene_239456 Copy
Species: Other
Genetic Insert: tyrosinase
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR, Cell-free system using bacterial extract; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39095367
Proper citation: RRID:Addgene_239454 Copy
Species: Other
Genetic Insert: 35S terminator seqeunce
Vector Backbone Description: Backbone Size:2222; Vector Backbone:pICH41276; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41351300
Comments: Please visit https://doi.org/10.1101/2025.06.20.660755 for bioRxiv preprint.
Proper citation: RRID:Addgene_210766 Copy
Species: Other
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:5700; Vector Backbone:RT14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41131151
Comments: Please visit https://doi.org/10.1101/2024.07.21.604473 for bioRxiv preprint.
Proper citation: RRID:Addgene_237445 Copy
Species: Other
Genetic Insert: spCas9-T2A-eBFP2
Vector Backbone Description: Backbone Marker:Fheng Zhang (Addgene #48138); Vector Backbone:PX458; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_233090 Copy
Species: Other
Genetic Insert: mWasabi
Vector Backbone Description: Vector Backbone:pIRL2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39618159
Proper citation: RRID:Addgene_227430 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pIRL2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39618159
Proper citation: RRID:Addgene_227431 Copy
Species: Other
Genetic Insert: mWasabi
Vector Backbone Description: Vector Backbone:pIRL117; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39618159
Proper citation: RRID:Addgene_227428 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:4859; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_200930 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:4859; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_200931 Copy
Species: Other
Genetic Insert: ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
Vector Backbone Description: Backbone Marker:Adam Karpf (Addgene #87360); Vector Backbone:TLCV2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39463974
Proper citation: RRID:Addgene_234948 Copy
Species: Other
Genetic Insert: ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
Vector Backbone Description: Backbone Marker:Adam Karpf (Addgene #87360); Vector Backbone:TLCV2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39463974
Proper citation: RRID:Addgene_234949 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:4859; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_200933 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:4859; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_200935 Copy
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