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Species: Homo sapiens
Genetic Insert: CLASP2 (340-1362) 9xS/A
Vector Backbone Description: Backbone Marker:Stratagene, ATCC; Backbone Size:33414; Vector Backbone:pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.
Due to difficulty in sequencing this plasmid, the EGFP and C-terminus of CLASP2 has not been sequence verified.
EGFP-CLASP2 340-1362 9xS/A was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_24523 Copy
Species: Homo sapiens
Genetic Insert: CLASP2 (340-1362)
Vector Backbone Description: Backbone Marker:Stratagene, ATCC; Backbone Size:33414; Vector Backbone:pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.
EGFP-CLASP2 340-1362 was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_24522 Copy
Species: Aequorea victoria and Discosoma sp. reef coral
Genetic Insert: Split RFP-GFP chimera
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20352097
Proper citation: RRID:Addgene_24515 Copy
Species: Aequorea victoria and Discosoma sp. reef coral
Genetic Insert: Split GFP-RFP chimera
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20352097
Proper citation: RRID:Addgene_24514 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
Clinical, molecular, and genotype-phenotype correlation studies from 25 cases of oral-facial-digital syndrome type 1: a French and Belgian collaborative study.
Thauvin-Robinet C, Cossée M, Cormier-Daire V, Van Maldergem L, Toutain A, Alembik Y, Bieth E, Layet V, Parent P, David A, Goldenberg A, Mortier G, Héron D, Sagot P, Bouvier AM, Huet F, Cusin V, Donzel A, Devys D, Teyssier JR, Faivre L.
J Med Genet. 2006 Jan;43(1):54-61.PMID: 16397067
Proper citation: RRID:Addgene_24581 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells.
Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA.
J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504
Proper citation: RRID:Addgene_24580 Copy
Species: C. glutamicum
Genetic Insert: TetRO promoter
Vector Backbone Description: Backbone Size:6078; Vector Backbone:pSHKLx; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15687380
Proper citation: RRID:Addgene_24730 Copy
Species: Bacterial
Genetic Insert: PAg85a-TetR
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pMV206; Vector Types:Mycobacterial shuttle vector; Bacterial Resistance:Kanamycin
Comments: There are a few mismatches between depositor's sequence and the quality control sequence Addgene has obtained. The region of mismatch and gaps is derived from the parental vector and not the important part of the vector.
Infect Immun. 2001 Dec;69(12):7851-7.
Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation.
Bateman BT, Donegan NP, Jarry TM, Palma M, Cheung AL.
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Abstract
An inducible promoter system provides a powerful tool for studying the genetic basis for virulence. A variety of inducible systems have been used in other organisms, including pXyl-xylR-inducible promoter, the pSpac-lacI system, and the arabinose-inducible P(BAD) promoter, but each of these systems has limitations in its application to Staphylococcus aureus. In this study, we demonstrated the efficacy of a tetracycline-inducible promoter system in inducing gene expression in S. aureus in vitro and inside epithelial cells as well as in an animal model of infection. Using the xyl/tetO promoter::gfp(uvr) fusion carried on a shuttle plasmid, we demonstrated that dose-dependent tetracycline induction, as measured by bacterial fluorescence, occurred in each of the above environments while basal activation under noninduced conditions remained low. To ascertain how the system can be used to elucidate the genetic basis of a pathogenic phenotype, we cloned the sigB gene downstream of the inducible promoter. Induction of SigB expression led to dose-dependent attachment of the tested strain to polystyrene microtiter wells. Additionally, bacterial microcolony formation, an event preceding mature biofilm formation, also increased with tetracycline induction of SigB.
PMID: 11705967 [PubMed - indexed for MEDLINE]PMCID: PMC98881Free PMC Articl
Proper citation: RRID:Addgene_24784 Copy
Species: synthetic
Genetic Insert: Prb
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21458342
Proper citation: RRID:Addgene_24785 Copy
Genetic Insert: Ptet-Gfp-ASV
Vector Backbone Description: Backbone Size:0; Vector Backbone:pFLAME4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15933004
Comments: 1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed]
2.
2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed]
Proper citation: RRID:Addgene_24782 Copy
Genetic Insert: Ptet-Gfp-LAA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pFLAME3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15933004
Comments: 1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed]
2.
2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed]
Proper citation: RRID:Addgene_24781 Copy
Species: Pyrococcus horikoshii
Genetic Insert: PH1109
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21458342
Proper citation: RRID:Addgene_24830 Copy
Genetic Insert: NpuDnaE-IntC
Vector Backbone Description: Backbone Size:3793; Vector Backbone:pRSF-1b (Invitrogen); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20203672
Proper citation: RRID:Addgene_24765 Copy
Species: Bacterial
Genetic Insert: TetR
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pMV206; Vector Types:Mycobacterial shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15933004
Comments: There are a few mismatches between depositor's sequence and the quality control sequence Addgene has obtained. The region of mismatch and gaps is derived from the parental vector and not the important part of the vector.
Infect Immun. 2001 Dec;69(12):7851-7.
Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation.
Bateman BT, Donegan NP, Jarry TM, Palma M, Cheung AL.
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Abstract
An inducible promoter system provides a powerful tool for studying the genetic basis for virulence. A variety of inducible systems have been used in other organisms, including pXyl-xylR-inducible promoter, the pSpac-lacI system, and the arabinose-inducible P(BAD) promoter, but each of these systems has limitations in its application to Staphylococcus aureus. In this study, we demonstrated the efficacy of a tetracycline-inducible promoter system in inducing gene expression in S. aureus in vitro and inside epithelial cells as well as in an animal model of infection. Using the xyl/tetO promoter::gfp(uvr) fusion carried on a shuttle plasmid, we demonstrated that dose-dependent tetracycline induction, as measured by bacterial fluorescence, occurred in each of the above environments while basal activation under noninduced conditions remained low. To ascertain how the system can be used to elucidate the genetic basis of a pathogenic phenotype, we cloned the sigB gene downstream of the inducible promoter. Induction of SigB expression led to dose-dependent attachment of the tested strain to polystyrene microtiter wells. Additionally, bacterial microcolony formation, an event preceding mature biofilm formation, also increased with tetracycline induction of SigB.
PMID: 11705967 [PubMed - indexed for MEDLINE]PMCID: PMC98881Free PMC Articl
Proper citation: RRID:Addgene_24779 Copy
Species: Danio rerio
Genetic Insert: Rab8B
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences.
Proper citation: RRID:Addgene_24908 Copy
Species: Danio rerio
Genetic Insert: fishRab8B
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences.
Proper citation: RRID:Addgene_24907 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24749 Copy
Species: Homo sapiens
Genetic Insert: Rabin8
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Proper citation: RRID:Addgene_24910 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24738 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24735 Copy
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