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Species: Homo sapiens
Genetic Insert: Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGAGATGGGCAGACGGAT-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTCATCATCTTCTCCCTCATC-3'.
Proper citation: RRID:Addgene_16175 Copy
Species: Homo sapiens
Genetic Insert: Polyglutamine binding protein 1
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGCCGCTGCCCGTTGCG-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG ATCCTGCTGCTTGGTTCGG-3'.
Proper citation: RRID:Addgene_16174 Copy
Species: Homo sapiens
Genetic Insert: BEST4
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_161678 Copy
Species: Homo sapiens
Genetic Insert: PANX2
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_161697 Copy
Species: Homo sapiens
Genetic Insert: solute carrier family 7 member 11
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:12000; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231310
Proper citation: RRID:Addgene_161819 Copy
Species: Homo sapiens
Genetic Insert: H2B-mTagBFP2
Vector Backbone Description: Backbone Marker:Malcolm Moore; Backbone Size:8326; Vector Backbone:pUltra-hot (Addgene Plasmid #24130); Vector Types:Mammalian Expression, Lentiviral, Multicistronic; Bacterial Resistance:Ampicillin
Comments: pUltra-hot-H2B-mTagBFP2 was cloned by Jorge L. Arias-Arias at Universidad de Costa Rica ([email protected]).
This vector allows the multicistronic expression of mCherry, H2B-mTagBFP2, and one gene of interest in mammalian cells. The construct contains two 2A peptides coding sequences and two cloning positions flanked by restriction sites:
Position 1 (5' XbaI – 3' BamHI): Occupied by the H2B-mTagBFP2 insert.
Position 2 (5' NheI – 3' EcoRI or SaII): Available for cloning one gene of interest.
The vector is suitable for transient transfection, but could also be used to produce lentiviral particles for stable expression in mammalian cells. For lentivirus packaging use the additional plasmids psPAX2 (Addgene Plasmid #12260) and pMD2.G (Addgene Plasmid #12259).
Proper citation: RRID:Addgene_161792 Copy
Species: Homo sapiens
Genetic Insert: HMG-box transcription factor 1
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGTGTGGGAAGTGAAGAC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG ATGTTGTTGTGAGCCTGAATTG-3'.
Proper citation: RRID:Addgene_16178 Copy
Species: Homo sapiens
Genetic Insert: Quaking
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGTCGGGGAAATGGAAAC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTTGCCGGTGGCGGCTCG-3'.
Proper citation: RRID:Addgene_16183 Copy
Species: Homo sapiens
Genetic Insert: Apoptosis-inducing factor
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGTTCCGGTGTGGAGGCC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTCTTCATGAATGTTGAATAGTT-3'.
Proper citation: RRID:Addgene_16182 Copy
Species: Homo sapiens
Genetic Insert: PP2A regulatory subunit B, beta isoform
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGAGGAGGACATTGATACC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTTAACCTTGTCCTGGAATATA-3'.
Proper citation: RRID:Addgene_16181 Copy
Species: Homo sapiens
Genetic Insert: PRKC gamma
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGCTGGTCTGGGCCCC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG CATGACGGGCACAGGCACT-3'.
Proper citation: RRID:Addgene_16180 Copy
Species: Homo sapiens
Genetic Insert: MAP7 (only amino acids 1-316)(includes MTBD and P123 domains)
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159728 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (full length, 1-963 amino acids), K963
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159727 Copy
Species: Homo sapiens
Genetic Insert: TFAM Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human TFAM in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_159745 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pFUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33093202
Comments: Please visit https://doi.org/10.1101/2020.07.31.231746 for bioRxiv preprint.
Proper citation: RRID:Addgene_159762 Copy
Species: Homo sapiens
Genetic Insert: spacer of sgRNA targeting FANCF gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32351707
Proper citation: RRID:Addgene_159786 Copy
Species: Homo sapiens
Genetic Insert: RPL10a
Vector Backbone Description: Backbone Size:6909; Vector Backbone:pSicoR-eIF1a-mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209477
Proper citation: RRID:Addgene_159890 Copy
Species: Homo sapiens
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590437
Comments: The PH domain of AKT1 has been deleted.
Proper citation: RRID:Addgene_15989 Copy
Species: Homo sapiens
Genetic Insert: CMV-OsTIR1(F74A)
Vector Backbone Description: Vector Backbone:pMK359; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_159992 Copy
Species: Homo sapiens
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: Targets collybistin MTL-CBSH3- mRNA (target is located in the protein coding region. Putative rat sequence based on human sequence - targets nucleotides 813-834 of human NM_001353924.2. Targets MTL collybistin isoforms without SH3 domain). There is also the mutated shRNA control for this isoform (Addgene #160211).
Proper citation: RRID:Addgene_160210 Copy
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