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Species: Synthetic
Genetic Insert: 7/8 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180724 Copy
Species: Synthetic
Genetic Insert: Int.1 Spacer
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180738 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34767345
Comments: Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180547 Copy
Species: Synthetic
Genetic Insert: S/1 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180710 Copy
Species: Synthetic
Genetic Insert: 1/2 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180712 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180674 Copy
Species: Synthetic
Genetic Insert: 5/E Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180719 Copy
Species: Synthetic
Genetic Insert: 2/E Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180713 Copy
Species: Synthetic
Genetic Insert: 2/3 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180714 Copy
Species: Synthetic
Genetic Insert: 3/4 Spacer Cassette
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180716 Copy
Species: Synthetic
Genetic Insert: tdTomato sgRNA
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:8652; Vector Backbone:PB_rtTA_BsmBI; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35789697
Comments: This plasmid was cloned with the one step BsmBI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
Proper citation: RRID:Addgene_188488 Copy
Species: Synthetic
Genetic Insert: Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
Vector Backbone Description: Backbone Size:2680; Vector Backbone:puc19; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985288
Proper citation: RRID:Addgene_162522 Copy
Species: Synthetic
Genetic Insert: Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
Vector Backbone Description: Backbone Size:2680; Vector Backbone:puc19; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985288
Proper citation: RRID:Addgene_162525 Copy
Species: Synthetic
Genetic Insert: Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
Vector Backbone Description: Backbone Size:2680; Vector Backbone:puc19; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985288
Proper citation: RRID:Addgene_162524 Copy
Species: Synthetic
Genetic Insert: Int.5 Spacer
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180742 Copy
Species: Synthetic
Genetic Insert: Int.7 Spacer
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180744 Copy
Species: Synthetic
Genetic Insert: Int.3 Spacer
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180740 Copy
Species: Synthetic
Genetic Insert: Int.4 Spacer
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180741 Copy
Species: Synthetic
Genetic Insert: dGFP
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739875
Proper citation: RRID:Addgene_176530 Copy
Species: Synthetic
Genetic Insert: Int.8 Spacer
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_180745 Copy
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