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Species: Rattus norvegicus
Genetic Insert: G protein alpha-o with constitutively activating R179C mutation
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17596375
Proper citation: RRID:Addgene_55780 Copy
Species: Homo sapiens
Genetic Insert: 3xFLAG tagged HERC2 (3550-4450aa)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24855649
Proper citation: RRID:Addgene_55788 Copy
Species: Homo sapiens
Genetic Insert: 3xFLAG tagged HERC2 (1700aa-2700aa)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24855649
Proper citation: RRID:Addgene_55786 Copy
Species: Homo sapiens
Genetic Insert: 3xFLAG tagged HERC2 (950aa-1750aa)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24855649
Proper citation: RRID:Addgene_55785 Copy
Species: Mus musculus
Genetic Insert: RIT2
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55663 Copy
Species: Mus musculus
Genetic Insert: alpha-q EE YFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNA1/amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17596375
Proper citation: RRID:Addgene_55782 Copy
Species: Homo sapiens
Genetic Insert: mCer(1-158)-beta5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNA1/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17596375
Comments: Reference for monomeric Ceulean:
Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_55708 Copy
Species: Mus musculus
Genetic Insert: RAP2C
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55668 Copy
Species: Mus musculus
Genetic Insert: RND1
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55701 Copy
Species: Homo sapiens
Genetic Insert: 3xFLAG tagged HERC2 (4421-4834aa)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24855649
Proper citation: RRID:Addgene_55789 Copy
Species: Mus musculus
Genetic Insert: DIRAS2
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55670 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2500; Vector Backbone:pENTR-1A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24841113
Comments: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.
Proper citation: RRID:Addgene_55791 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2500; Vector Backbone:pENTR-1A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24841113
Comments: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.
Proper citation: RRID:Addgene_55790 Copy
Species: Mus musculus
Genetic Insert: RASL11B
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55678 Copy
Species: Mus musculus
Genetic Insert: RASL10B
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55673 Copy
Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12623879
Comments: The distance between the two Not1 sites is ~6kb
Clone into the Sal1 site (or Sal/Hind3 site).
Release the pBluescript backbone with Not1 for transgenics.
This construct consists of a full-length α-MHC promoter in which 3 GATA sites and 2 TREs were ablated. Other cis-acting regions important for cardiac-specific expression were left intact. To make the attenuated promoter Tet responsive, 7 repeats of the TetO sequences were inserted adjacent to the TATA box. The human growth hormone polyadenylation signal was placed downstream from the unique cloning site. Any gene of interest inserted into this Tet-responsive promoter construct will be inducible.
Proper citation: RRID:Addgene_55792 Copy
Species: Mus musculus
Genetic Insert: p110 beta
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55719 Copy
Species: Mus musculus
Genetic Insert: p110 alpha
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55713 Copy
Species: Mus musculus
Genetic Insert: GEM
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Proper citation: RRID:Addgene_55680 Copy
Species: Mus musculus
Genetic Insert: p110 alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pBacPAK; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23706742
Comments: murine p110α and p110β constructs (wild type and RBD DM) subcloned into pBacPAK-His3-GST (from protein purification facility). So these constructs encode GST-tagged p110, which can be cleaved of using rhinovirus 3C protease
Proper citation: RRID:Addgene_55722 Copy
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