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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAdEasy-EGFP-CLASP2 340-1362 9xS/A Resource Report Resource Website |
RRID:Addgene_24523 | CLASP2 (340-1362) 9xS/A | Homo sapiens | Kanamycin | PMID:19289791 | See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for. Due to difficulty in sequencing this plasmid, the EGFP and C-terminus of CLASP2 has not been sequence verified. EGFP-CLASP2 340-1362 9xS/A was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1. AdEasy is a registered trademark of the Johns Hopkins University. | Backbone Marker:Stratagene, ATCC; Backbone Size:33414; Vector Backbone:pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin | Nonphosphorylatable CLASP2 deletion mutant. Mutation of serines in GSK3β motif between S594 to S614 plus S568/572/576A. | 2026-08-15 01:12:33 | 0 |
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pAdEasy-EGFP-CLASP2 340-1362 Resource Report Resource Website |
RRID:Addgene_24522 | CLASP2 (340-1362) | Homo sapiens | Kanamycin | PMID:19289791 | See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for. EGFP-CLASP2 340-1362 was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1. AdEasy is a registered trademark of the Johns Hopkins University. | Backbone Marker:Stratagene, ATCC; Backbone Size:33414; Vector Backbone:pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin | CLASP2 deletion mutant that retains MT lattice binding activity, MT plus end tracking activity and C-terminal domain. | 2026-08-15 01:12:31 | 0 |
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pENTR-Site6 Resource Report Resource Website |
RRID:Addgene_24515 | Split RFP-GFP chimera | Aequorea victoria and Discosoma sp. reef coral | Kanamycin | PMID:20352097 | Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:31 | 0 | ||
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pENTR-Site5 Resource Report Resource Website |
RRID:Addgene_24514 | Split GFP-RFP chimera | Aequorea victoria and Discosoma sp. reef coral | Kanamycin | PMID:20352097 | Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:31 | 0 | ||
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pIRES2-eGFP-Ofd1-Myc-G139S Resource Report Resource Website |
RRID:Addgene_24581 | Ofd1 | Mus musculus | Kanamycin | PMID:20230748 | Mutation reported in: Clinical, molecular, and genotype-phenotype correlation studies from 25 cases of oral-facial-digital syndrome type 1: a French and Belgian collaborative study. Thauvin-Robinet C, Cossée M, Cormier-Daire V, Van Maldergem L, Toutain A, Alembik Y, Bieth E, Layet V, Parent P, David A, Goldenberg A, Mortier G, Héron D, Sagot P, Bouvier AM, Huet F, Cusin V, Donzel A, Devys D, Teyssier JR, Faivre L. J Med Genet. 2006 Jan;43(1):54-61.PMID: 16397067 | Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Glycine 139 changed to serine (codon GGT to AGT) | 2026-08-15 01:12:32 | 0 |
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pIRES2-eGFP-Ofd1-Myc-S75F Resource Report Resource Website |
RRID:Addgene_24580 | Ofd1 | Mus musculus | Kanamycin | PMID:20230748 | Mutation reported in: OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells. Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA. J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504 | Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | serine 75 changed to phenylalanine (codon TCT changed to TTT) | 2026-08-15 01:12:33 | 0 |
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pMIND Resource Report Resource Website 1+ mentions |
RRID:Addgene_24730 | TetRO promoter | C. glutamicum | Kanamycin | PMID:15687380 | Backbone Size:6078; Vector Backbone:pSHKLx; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:35 | 2 | ||
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pTACT13 Resource Report Resource Website |
RRID:Addgene_24784 | PAg85a-TetR | Bacterial | Kanamycin | There are a few mismatches between depositor's sequence and the quality control sequence Addgene has obtained. The region of mismatch and gaps is derived from the parental vector and not the important part of the vector. Infect Immun. 2001 Dec;69(12):7851-7. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Bateman BT, Donegan NP, Jarry TM, Palma M, Cheung AL. Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. Abstract An inducible promoter system provides a powerful tool for studying the genetic basis for virulence. A variety of inducible systems have been used in other organisms, including pXyl-xylR-inducible promoter, the pSpac-lacI system, and the arabinose-inducible P(BAD) promoter, but each of these systems has limitations in its application to Staphylococcus aureus. In this study, we demonstrated the efficacy of a tetracycline-inducible promoter system in inducing gene expression in S. aureus in vitro and inside epithelial cells as well as in an animal model of infection. Using the xyl/tetO promoter::gfp(uvr) fusion carried on a shuttle plasmid, we demonstrated that dose-dependent tetracycline induction, as measured by bacterial fluorescence, occurred in each of the above environments while basal activation under noninduced conditions remained low. To ascertain how the system can be used to elucidate the genetic basis of a pathogenic phenotype, we cloned the sigB gene downstream of the inducible promoter. Induction of SigB expression led to dose-dependent attachment of the tested strain to polystyrene microtiter wells. Additionally, bacterial microcolony formation, an event preceding mature biofilm formation, also increased with tetracycline induction of SigB. PMID: 11705967 [PubMed - indexed for MEDLINE]PMCID: PMC98881Free PMC Articl | Backbone Size:4120; Vector Backbone:pMV206; Vector Types:Mycobacterial shuttle vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:34 | 0 | ||
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Prb Resource Report Resource Website |
RRID:Addgene_24785 | Prb | synthetic | Kanamycin | PMID:21458342 | Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:34 | 0 | ||
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pHLEGM3 Resource Report Resource Website |
RRID:Addgene_24782 | Ptet-Gfp-ASV | Kanamycin | PMID:15933004 | 1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed] 2. 2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed] | Backbone Size:0; Vector Backbone:pFLAME4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:34 | 0 | ||
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pHLEGM2 Resource Report Resource Website |
RRID:Addgene_24781 | Ptet-Gfp-LAA | Kanamycin | PMID:15933004 | 1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed] 2. 2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed] | Backbone Size:0; Vector Backbone:pFLAME3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:34 | 0 | ||
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PH1109 Resource Report Resource Website |
RRID:Addgene_24830 | PH1109 | Pyrococcus horikoshii | Kanamycin | PMID:21458342 | Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:34 | 0 | ||
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pHYRSF1-02 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24765 | NpuDnaE-IntC | Kanamycin | PMID:20203672 | Backbone Size:3793; Vector Backbone:pRSF-1b (Invitrogen); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:35 | 3 | |||
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pTACT1 Resource Report Resource Website |
RRID:Addgene_24779 | TetR | Bacterial | Kanamycin | PMID:15933004 | There are a few mismatches between depositor's sequence and the quality control sequence Addgene has obtained. The region of mismatch and gaps is derived from the parental vector and not the important part of the vector. Infect Immun. 2001 Dec;69(12):7851-7. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Bateman BT, Donegan NP, Jarry TM, Palma M, Cheung AL. Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. Abstract An inducible promoter system provides a powerful tool for studying the genetic basis for virulence. A variety of inducible systems have been used in other organisms, including pXyl-xylR-inducible promoter, the pSpac-lacI system, and the arabinose-inducible P(BAD) promoter, but each of these systems has limitations in its application to Staphylococcus aureus. In this study, we demonstrated the efficacy of a tetracycline-inducible promoter system in inducing gene expression in S. aureus in vitro and inside epithelial cells as well as in an animal model of infection. Using the xyl/tetO promoter::gfp(uvr) fusion carried on a shuttle plasmid, we demonstrated that dose-dependent tetracycline induction, as measured by bacterial fluorescence, occurred in each of the above environments while basal activation under noninduced conditions remained low. To ascertain how the system can be used to elucidate the genetic basis of a pathogenic phenotype, we cloned the sigB gene downstream of the inducible promoter. Induction of SigB expression led to dose-dependent attachment of the tested strain to polystyrene microtiter wells. Additionally, bacterial microcolony formation, an event preceding mature biofilm formation, also increased with tetracycline induction of SigB. PMID: 11705967 [PubMed - indexed for MEDLINE]PMCID: PMC98881Free PMC Articl | Backbone Size:4120; Vector Backbone:pMV206; Vector Types:Mycobacterial shuttle vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:35 | 0 | |
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pENTR-fishRab8B[Q67L] Resource Report Resource Website |
RRID:Addgene_24908 | Rab8B | Danio rerio | Kanamycin | PMID:17574030 | This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences. | Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Changed Glutamine 67 to Leucine | 2026-08-15 01:12:36 | 0 |
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pENTR-fishRab8B Resource Report Resource Website |
RRID:Addgene_24907 | fishRab8B | Danio rerio | Kanamycin | PMID:17574030 | This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences. | Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:34 | 0 | |
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pDONR221-hSOX10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24749 | (sex determining region Y)-box 10 | Homo sapiens | Kanamycin | PMID:19422606 | Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | wildtype sequence | 2026-08-15 01:12:33 | 6 | |
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pENTR-Rabin8 Resource Report Resource Website |
RRID:Addgene_24910 | Rabin8 | Homo sapiens | Kanamycin | PMID:17574030 | Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:12:34 | 0 | ||
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pDONR221-hSOX10-G1238A Resource Report Resource Website |
RRID:Addgene_24738 | (sex determining region Y)-box 10 | Homo sapiens | Kanamycin | PMID:19422606 | Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | G replaced with A at position 1238 resulting in G413D. | 2026-08-15 01:12:33 | 0 | |
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pDONR221-hSOX10-C1240T Resource Report Resource Website |
RRID:Addgene_24735 | (sex determining region Y)-box 10 | Homo sapiens | Kanamycin | PMID:19422606 | Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | C replaced with T at position 1240 resulting in H414Y. | 2026-08-15 01:12:33 | 0 |
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