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Species: Mus musculus
Genetic Insert: mouse myc ULK1
Vector Backbone Description: Backbone Size:7341; Vector Backbone:pcDNA 6.2
V5
dest
(Invitrogen); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205641
Comments: C-term V5 not expressed because of stop codon at end of ULK1.
Proper citation: RRID:Addgene_27629 Copy
Species: Mus musculus
Genetic Insert: Pax3
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7744814
Proper citation: RRID:Addgene_27319 Copy
Species: Mus musculus
Genetic Insert: Taz
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_27318 Copy
Species: Mus musculus
Genetic Insert: Cortactin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Adapted from Natalie Soulet Cortactin-pEGFP-C1 construct.
Proper citation: RRID:Addgene_27676 Copy
Species: Mus musculus
Genetic Insert: OCRL1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 30533468 (MGC:93022).
Proper citation: RRID:Addgene_27675 Copy
Species: Mus musculus
Genetic Insert: protein kinase B gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6157; Vector Backbone:pcDNA3.1/CT-GFP-Topo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The GFP is not expressed as there is a stop codon after the HA tag.
mTORC2 reportedly can phosphorylate FSY and FSF sites, so the Y473F mutation should not affect expression or function.
Proper citation: RRID:Addgene_27293 Copy
Species: Mus musculus
Genetic Insert: Cofilin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Gene PCR from cDNA library from mouse brain
Proper citation: RRID:Addgene_27687 Copy
Species: Mus musculus
Genetic Insert: CIP4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 3500118 (MGC:6673).
Proper citation: RRID:Addgene_27685 Copy
Species: Mus musculus
Genetic Insert: Ack1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 6400209 (MGC:63428).
Proper citation: RRID:Addgene_27684 Copy
Species: Mus musculus
Genetic Insert: Clathrin Light Chain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 3154277 (MGC:68133).
Proper citation: RRID:Addgene_27680 Copy
Species: Mus musculus
Genetic Insert: cavin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5069; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20070607
Proper citation: RRID:Addgene_27709 Copy
Species: Mus musculus
Genetic Insert: Prkar1a
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pMT-REV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8388994
Comments: Expression vector encoding dominant-negative regulatory subunit of type 1 cAMP-dependent protein kinase.
pMT-REV is a zinc-inducible expression vector driven by the metallothionein promoter.
Proper citation: RRID:Addgene_28179 Copy
Species: Mus musculus
Genetic Insert: Abp1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5400; Vector Backbone:pEGFP-N1 (EGFP replaced with tDimer-RFP); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 4986658 (MGC:51371).
Cloned using Gateway acceptance vectors.
Proper citation: RRID:Addgene_27699 Copy
Species: Mus musculus
Genetic Insert: IL6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17606866
Proper citation: RRID:Addgene_28088 Copy
Species: Mus musculus
Genetic Insert: DJ1
Vector Backbone Description: Backbone Size:4756; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15663963
Proper citation: RRID:Addgene_29396 Copy
Species: Mus musculus
Genetic Insert: Oct4, Sox2, Klf4, c-Myc, Lin28
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21243013
Comments: Please note that Addgene's sequencing results using the CMV-F and pCEP-F primers found 2 sequence variants within and after the CMV enhancer. One is a C->G mismatch at position 775 within the CMV enhancer and the second is a deletion at position 949 just after the end of the CAG enhancer, as compared to the full plasmid sequence provided by the depositing scientist. The significance of the two sequence variants is unclear; however, the plasmid functions as described in the associated publication. Addgene's other 4 sequencing results completely matched the depositor's sequence.
Proper citation: RRID:Addgene_28213 Copy
Species: Mus musculus
Genetic Insert: NHERF1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV-2-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17242191
Proper citation: RRID:Addgene_28297 Copy
Species: Mus musculus
Genetic Insert: integrin beta 1
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18160654
Proper citation: RRID:Addgene_30153 Copy
Species: Mus musculus
Genetic Insert: Bmi1
Vector Backbone Description: Backbone Size:4918; Vector Backbone:pCS2+ att-cherry-HA (C-term cherry-HA); Vector Types:Mammalian Expression, Xenopus/Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552206
Proper citation: RRID:Addgene_30516 Copy
Species: Mus musculus
Genetic Insert: macroH2A1.2-C
Vector Backbone Description: Vector Backbone:pDEST pCS2+ att-eGFP-HA (C term eGFP-HA); Vector Types:Mammalian Expression, Xenopus/Zebrafish expression.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552206
Proper citation: RRID:Addgene_30515 Copy
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