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Genetic Insert: None
Vector Backbone Description: Vector Backbone:pCAndy; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24550272
Proper citation: RRID:Addgene_53571 Copy
Species: Homo sapiens
Genetic Insert: RECK-3'UTR
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22761427
Proper citation: RRID:Addgene_53614 Copy
Species: Other
Genetic Insert: BRPF1B
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4LC2/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53620 Copy
Species: Homo sapiens
Genetic Insert: TPP1
Vector Backbone Description: Backbone Marker:Invitrogen/LIfe Technologies; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22863003
Proper citation: RRID:Addgene_53585 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyE
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK-; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8837512
Proper citation: RRID:Addgene_53586 Copy
Species: Other
Genetic Insert: JMJD1BA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/4C8D/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53627 Copy
Species: Other
Genetic Insert: BAZ2AA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4LZ2/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53623 Copy
Species: Synthetic
Genetic Insert: Nano-lantern-based luminescent cAMP indicator
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23232392
Proper citation: RRID:Addgene_53594 Copy
Species: Homo sapiens
Genetic Insert: CYYR1
Vector Backbone Description: Backbone Marker:Lawson Lab (Addgene Plasmid 22423); Backbone Size:4095; Vector Backbone:pCSDest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29760202
Comments: BC036761
Proper citation: RRID:Addgene_53794 Copy
Species: Homo sapiens
Genetic Insert: SAMSN1
Vector Backbone Description: Backbone Marker:Lawson Lab (Addgene Plasmid 22423); Backbone Size:4095; Vector Backbone:pCSDest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29760202
Comments: NM_022136
Proper citation: RRID:Addgene_53792 Copy
Species: E. coli
Genetic Insert: αNTD
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121589
Comments: This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53731 Copy
Species: Human papillomavirus
Genetic Insert: HPV16-E7
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24708873
Proper citation: RRID:Addgene_53696 Copy
Species: Human papillomavirus
Genetic Insert: HPV16-E1(partial)
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24708873
Proper citation: RRID:Addgene_53699 Copy
Species: Other
Genetic Insert: λCI-β-flap fusion
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:9121589
Comments: Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details.
This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53733 Copy
Species: Homo sapiens
Genetic Insert: Wilms tumor 1 associated protein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316715
Proper citation: RRID:Addgene_53741 Copy
Species: Other
Genetic Insert: 2x membrane linker
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19378112
Comments: Use NotI/SP6 for RNA to inject.
Put XbaI/AgeI membrane linker in place of histone2B in XbaI/AgeI cut pCS-H2Bcerulean.
This puts membrane-cerulean between XbaI and SnaBI of pCS.
Proper citation: RRID:Addgene_53749 Copy
Species: Homo sapiens
Genetic Insert: histone H2B
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19378112
Comments: Cut with NotI and use SP6 for sense RNA.
Proper citation: RRID:Addgene_53744 Copy
Species: Homo sapiens
Genetic Insert: Sec8
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24389102
Proper citation: RRID:Addgene_53758 Copy
Species: Homo sapiens
Genetic Insert: Sec10
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24389102
Proper citation: RRID:Addgene_53759 Copy
Species: Homo sapiens
Genetic Insert: Sec5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24389102
Proper citation: RRID:Addgene_53756 Copy
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