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Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34168320
Proper citation: RRID:Addgene_167158 Copy
Species: Homo sapiens
Genetic Insert: DUSP6
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23839489
Comments: Catalytically inactive DUSP6
Proper citation: RRID:Addgene_27977 Copy
Species: Homo sapiens
Genetic Insert: DUSP6
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:11000; Vector Backbone:pLEX-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23839489
Proper citation: RRID:Addgene_27975 Copy
Species: Homo sapiens
Genetic Insert: SC4MOL
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Comments: H173Q caused by 519T->A mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510
Proper citation: RRID:Addgene_27984 Copy
Species: Homo sapiens
Genetic Insert: SC4MOL
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_27983 Copy
Vector Backbone Description: Backbone Size:8493; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:11483365
Comments: Note that Addgene's sequencing results found a 1 nucleotide deletion at bp# 4286 when compared with the sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.
Proper citation: RRID:Addgene_28035 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH10
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40861 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH6
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40857 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH4
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40855 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH2
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40853 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH8
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40859 Copy
Species: bacteria
Genetic Insert: Promotorless gfp reporter gene
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:11059491
Comments: Please refer to the attached table for a complete list of restriction sites in the MCS.
This plasmid has the PstI site.
Proper citation: RRID:Addgene_37822 Copy
Species: bacteria
Genetic Insert: Promotorless gfp reporter gene
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:11059491
Comments: Please refer to the attached table for a complete list of restriction sites in the MCS.
This plasmid has the PstI site.
Proper citation: RRID:Addgene_37823 Copy
Species: Enterobacteria phage P1
Genetic Insert: cre recombinase
Vector Backbone Description: Backbone Marker:Lidstrom Lab (Addgene plasmid 45826); Backbone Size:6878; Vector Backbone:pCM62; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12449384
Comments: The 1.1-kb XbaI-EcoRI fragment from pJW168 (PMID 9858684) was cloned between the XbaI and EcoRI sites of pCM62 (Addgene plasmid 45826) to generate the tetracycline-resistance conferring cre expression plasmid.
Used with allelic exhange vectors--Addgene plasmids 46012 or 46013--to generate unmarked mutant strains in a broad bacterial host range.
Proper citation: RRID:Addgene_45863 Copy
Vector Backbone Description: Backbone Marker:Lidstrom Lab (Addgene plasmid 45826); Backbone Size:7965; Vector Backbone:pCM62; Vector Types:low-background broad-host-range promoter-probe vector using xylE as a reporter gene; Bacterial Resistance:Tetracycline
Defining Citation: PMID:11495985
Comments: XylE replaces the lac promoter in pCM62 (Addgene plasmid 45826) and is used as a reporter to test promoter activity. A transcriptional terminator was added to reduce background activity.
Proper citation: RRID:Addgene_45828 Copy
Vector Backbone Description: Backbone Size:7395; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24050148
Comments: Reference:
St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j
Proper citation: RRID:Addgene_45989 Copy
Vector Backbone Description: Vector Backbone:original; Vector Types:Bacterial targeting; Bacterial Resistance:Tetracycline
Defining Citation: PMID:10337469
Proper citation: RRID:Addgene_46259 Copy
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5595; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_46974 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 43829; Backbone Size:5173; Vector Backbone:pRE107 (modified from pGP704); Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Tetracycline
Defining Citation: PMID:9511756
Comments: The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination.
Plasmid pRE107 (Addgene plasmid #43829) was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis.
To use this plasmid with bla fusions and increase the functionality of this system, the depositing laboratory replaced the ApR gene as follows.
The ApR gene flanked by the remaining BamHI sites was removed and replaced with either CmR, TcR or KmR resistance markers (see associated schematic image).
The TcR gene was cloned on a blunted EcoRI–StyI fragment from pBR322 into the blunted BamHI sites to give pDMS197. The BamHI site near the MCS was regenerated after cloning; the BamHI site near the 5' end of TcR/TetR was not.
The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers. Moreover, the use of Tc also allows an alternative negative selection using fusaric acid and chlortetracycline as described previously (Maloy and Nunn, 1981).
Proper citation: RRID:Addgene_43831 Copy
Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101314 Copy
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