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Species: Homo sapiens
Genetic Insert: utrophin
Vector Backbone Description: Backbone Size:12500; Vector Backbone:tol2:mpx-gfp; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20159593
Proper citation: RRID:Addgene_45247 Copy
Species: Homo sapiens
Genetic Insert: Cenp-B-DBD INCENP-Δcen mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21844210
Proper citation: RRID:Addgene_45233 Copy
Species: Homo sapiens
Genetic Insert: Cenp-B, INCENP-Δcen, mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21844210
Proper citation: RRID:Addgene_45235 Copy
Species: Homo sapiens
Genetic Insert: Cenp-B-DBD, INCENP-Δcen, GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21844210
Proper citation: RRID:Addgene_45237 Copy
Species: Homo sapiens
Genetic Insert: mCherry KNL1 resistant to KNL1 siRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20231380
Proper citation: RRID:Addgene_45224 Copy
Species: Homo sapiens
Genetic Insert: untargeted Plk1 FRET sensor Myt1 substrate
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18463638
Proper citation: RRID:Addgene_45217 Copy
Species: Homo sapiens
Genetic Insert: H2Afy2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1-CT-MYC-HIS-B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11331621
Comments: Please note that Addgene's sequencing result identified a single nucleotide mismatch at bp# 653 when compared to GenBank ID AF336304. This silent mutation does not affect the protein sequence.
Proper citation: RRID:Addgene_45170 Copy
Species: Homo sapiens
Genetic Insert: H2AFB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1-CT-MYC-A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11266453
Proper citation: RRID:Addgene_45172 Copy
Species: Homo sapiens
Genetic Insert: H2Afy2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11331621
Comments: Please note that Addgene's sequencing result identified a single nucleotide mismatch at bp# 653 when compared to GenBank ID AF336304. This silent mutation does not affect the protein sequence.
Proper citation: RRID:Addgene_45171 Copy
Species: Homo sapiens
Genetic Insert: H2AFB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6141; Vector Backbone:pcDNA3.1-CT-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11266453
Proper citation: RRID:Addgene_45173 Copy
Species: Homo sapiens
Genetic Insert: H2Afy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1-CT-MYC-HIS-A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11331621
Proper citation: RRID:Addgene_45166 Copy
Species: Homo sapiens
Genetic Insert: TLOC1
Vector Backbone Description: Vector Backbone:pLEX_970; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Comments: The initial pDONR223 clone was obtained from the CCSB Human Orfeome Collection. Upon sequencing it was found that the construct contained a 660 bp long variant as compared to the expected 1200 bp long version.
To determine whether this shorter version is expressed in cancer cells, and to exclude the possibility of this being a cloning artifact, we cloned TLOC1 from T47D cells and found both forms to be expressed. We re-cloned both the full length and shorter splice variants by ligation of BP flanked PCR products into pDONR223 by a Gateway BP reaction.
TLOC1 was transferred into DEST vectors from TLOC1 containing pDONR223 vectors through a Gateway LR reaction.
Proper citation: RRID:Addgene_45287 Copy
Species: Homo sapiens
Genetic Insert: H2Afy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3.1-CT-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11331621
Proper citation: RRID:Addgene_45169 Copy
Species: Homo sapiens
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9786947
Comments: The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis.
Frank S, Gaume B, Bergmann-Leitner ES, Leitner WW, Robert EG, Catez F, Smith CL, Youle RJ
In Dev Cell. 2001 Oct;1(4):515-25.
Proper citation: RRID:Addgene_45161 Copy
Species: Homo sapiens
Genetic Insert: pLAC-C25
Vector Backbone Description: Backbone Marker:New England Biolabs, INC; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression, Protein Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16923831
Comments: The last 25 amino acids of lacritin were deleted in this construct (aa 114-138).
Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence. The depositing laboratory indicates that these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_45283 Copy
Species: Homo sapiens
Genetic Insert: TNIK
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Comments: The TNIK open reading frame was PCR amplified with BP flanked primers from the vector acquired from Open Biosystems and ligated into a pDONR223 vector in a BP Gateway ligation reaction. The TNIK open reading frame was subsequently ligated into the pBabe_puro_DEST_flag backbone in a Gateway LR ligation reaction.
Proper citation: RRID:Addgene_45276 Copy
Species: Homo sapiens
Genetic Insert: LRRC31
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45271 Copy
Species: Homo sapiens
Genetic Insert: MCOPS3
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45270 Copy
Species: Homo sapiens
Genetic Insert: TLOC1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45273 Copy
Species: Homo sapiens
Genetic Insert: GPR160
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45274 Copy
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