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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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fox1 (a2bp1l)_L (OZ519) Resource Report Resource Website |
RRID:Addgene_27194 | Zinc finger array targeting fox1 (a2bp1l) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene fox1 (a2bp1l) (see below). Please note that this plasmid does NOT contain the fox1 (a2bp1l) sequence. Users must order the complementary plasmid fox1 (a2bp1l)_R (OZ520) [Addgene plasmid 27195] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGTC-3' in a bacterial two hybrid assay, and resulted in 7.12 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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pitrm1_R (OZ516) Resource Report Resource Website |
RRID:Addgene_27191 | Zinc finger array targeting pitrm1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene pitrm1 (see below). Please note that this plasmid does NOT contain the pitrm1 sequence. Users must order the complementary plasmid pitrm1_L (OZ515) [Addgene plasmid 27190] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGAAGAG-3' in a bacterial two hybrid assay, and resulted in 8.11 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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dpf2_R (OZ532) Resource Report Resource Website |
RRID:Addgene_27209 | Zinc finger array targeting dpf2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_L (OZ531) [Addgene plasmid 27208] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGAAACG-3' in a bacterial two hybrid assay, and resulted in 5.71 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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dpf2_L (OZ531) Resource Report Resource Website |
RRID:Addgene_27208 | Zinc finger array targeting dpf2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_R (OZ532) [Addgene plasmid 27209] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGTAGCA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:01 | 0 | |
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mdka (midkine-related growth factor)_R (OZ528) Resource Report Resource Website |
RRID:Addgene_27205 | Zinc finger array targeting mdka | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_L (OZ527) [Addgene plasmid 27204] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGGTGGC-3' in a bacterial two hybrid assay, and resulted in 3.75 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:01 | 0 | |
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mdka (midkine-related growth factor)_L (OZ527) Resource Report Resource Website |
RRID:Addgene_27204 | Zinc finger array targeting mdka | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_R (OZ528) [Addgene plasmid 27205] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAGGGT-3' in a bacterial two hybrid assay, and resulted in 7.20 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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pDestTol2pA2_ubi:EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_27323 | ubiquitin:EGFP reporter | Danio rerio | Ampicillin | PMID:21138979 | There are a few nucleotide discrepancies between author sequence and Addgene quality control sequence. According to the depositing lab, these do not affect plasmid function. | Backbone Size:4179; Vector Backbone:pDestTol2A2; Vector Types:Tol2 transposon; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:02 | 5 | |
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pENTR5'_ubi Resource Report Resource Website 10+ mentions |
RRID:Addgene_27320 | zebrafish ubiquitin promoter | Danio rerio | Kanamycin | PMID:21138979 | Backbone Marker:Invitrogen; Backbone Size:2692; Vector Backbone:pENTR5' TOPO; Vector Types:Multisite Gateway 5' entry vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:02 | 25 | ||
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pGEM-T ZfHoxc9a Resource Report Resource Website |
RRID:Addgene_27537 | Hoxc9a | Danio rerio | Ampicillin | PMID:9425136 | Addgene's quality control sequence has some mismatches with NCBI sequence for this gene; the mismatches should not affect plasmid function. | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:03 | 0 | |
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pGEM-T ZfHoxc8a Resource Report Resource Website |
RRID:Addgene_27538 | Hoxc8a | Danio rerio | Ampicillin | PMID:9425136 | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | ||
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pGEM-T ZfHoxd9a Resource Report Resource Website |
RRID:Addgene_27535 | Hoxd9a | Danio rerio | Ampicillin | PMID:9425136 | Addgene's quality control sequence has some mismatches with NCBI's sequence for this gene; the mismatches should not affect plasmid function. | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |
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pGEM-T ZfHoxb10a Resource Report Resource Website |
RRID:Addgene_27530 | Hoxb10a | Danio rerio | Ampicillin | PMID:9425136 | Addgene's quality control sequence has some mismatches with NCBI's sequence for this gene; the mismatches should not affect plasmid function. | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |
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pGEM-T ZfHoxd11a Resource Report Resource Website |
RRID:Addgene_27544 | Hoxd11a | Danio rerio | Ampicillin | Addgene's quality control sequence has some mismatches with NCBI's sequence for this gene; the mismatches should not affect plasmid function. | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | ||
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pGEM-T ZfHoxc11a Resource Report Resource Website |
RRID:Addgene_27542 | Hoxc11a | Danio rerio | Ampicillin | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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pBS ZfHoxa9a Resource Report Resource Website |
RRID:Addgene_27518 | Hoxa9a | Danio rerio | Ampicillin | PMID:9425136 | Addgene's quality control sequence has some mismatches with NCBI sequence for this gene; the mismatches should not affect plasmid function. | Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:03 | 0 | |
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pGEM-T ZfHoxa3a Resource Report Resource Website |
RRID:Addgene_27515 | Hoxa3a | Danio rerio | Ampicillin | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression, Riboprobe synthesis; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:03 | 0 | |||
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pGEM-T ZfHoxa4a Resource Report Resource Website |
RRID:Addgene_27516 | Hoxa4a | Danio rerio | Ampicillin | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression, Riboprobe synthesis; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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pBS ZfHoxb5a Resource Report Resource Website |
RRID:Addgene_27524 | Hoxb5a | Danio rerio | Ampicillin | Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:03 | 0 | |||
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pBS ZfHoxb3a Resource Report Resource Website |
RRID:Addgene_27522 | Hoxb3a | Danio rerio | Ampicillin | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK-; Vector Types:Riboprobe synthesis; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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pGEM-T ZfHoxb4a Resource Report Resource Website |
RRID:Addgene_27523 | Hoxb4a | Danio rerio | Ampicillin | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 |
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