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Species: Homo sapiens
Genetic Insert: shPOLE1_2
Vector Backbone Description: Backbone Marker:The RNAi Consortium; Backbone Size:7466; Vector Backbone:pLKO.1B; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33152268
Proper citation: RRID:Addgene_160765 Copy
Species: Homo sapiens
Genetic Insert: shCDC25A_1
Vector Backbone Description: Backbone Marker:The RNAi Consortium; Backbone Size:7466; Vector Backbone:pLKO.1B; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33152268
Proper citation: RRID:Addgene_160767 Copy
Species: Homo sapiens
Genetic Insert: shCDC25C_1
Vector Backbone Description: Backbone Marker:The RNAi Consortium; Backbone Size:7466; Vector Backbone:pLKO.1B; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33152268
Proper citation: RRID:Addgene_160769 Copy
Species: Homo sapiens
Genetic Insert: POLE1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pMXS-IRES-BLAST; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33152268
Proper citation: RRID:Addgene_160802 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q148A and a second mutagenesis to yield the double mutant.
Proper citation: RRID:Addgene_158633 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). This plamid has an additional Q271A mutation, and amino acid 93 (glutamate, E) was mutated back to the reference (glutamine, Q). Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q271A and a second mutagenesis to yield the double mutant. The clones displayed consistent off-target mutations at one of two points. Two clones with different off-target mutations were digested and the good pieces ligated together so to yield the final sequence.
Proper citation: RRID:Addgene_158632 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F. This plasmid has an additional Q148A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) on the pEN_TT 3XFLAG-MAVS plasmid.
Proper citation: RRID:Addgene_158630 Copy
Species: Homo sapiens
Genetic Insert: OAS2
Vector Backbone Description: Backbone Marker:Addgene #25896; Vector Backbone:pLX302; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33740397
Proper citation: RRID:Addgene_158642 Copy
Species: Homo sapiens
Genetic Insert: Mx1
Vector Backbone Description: Backbone Marker:Addgene #25896; Vector Backbone:pLX302; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33740397
Comments: Addgene NGS identified F660S variant in MX1 ORF, depositing lab confirmed this variant present in the original version of plasmid used in associated publication.
Proper citation: RRID:Addgene_158640 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Vector Backbone:pXPR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30305350
Comments: For questions regarding use of this plasmid, please contact John Doench.
Proper citation: RRID:Addgene_158704 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human SLC12A5 gene stop codon
Vector Backbone Description: Backbone Size:9300; Vector Backbone:Cas9-PolyA-CMV-GFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31366578
Proper citation: RRID:Addgene_158577 Copy
Species: Homo sapiens
Genetic Insert: eIF4G1 (with microexon)
Vector Backbone Description: Vector Backbone:pCDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31999954
Proper citation: RRID:Addgene_158776 Copy
Species: Homo sapiens
Genetic Insert: eIF4G1 (with microexon)
Vector Backbone Description: Backbone Marker:Blencowe/Gingras labs; Vector Backbone:pCDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31999954
Proper citation: RRID:Addgene_158790 Copy
Species: Homo sapiens
Genetic Insert: eIF4G1 (codon optimized)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pET SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31999954
Proper citation: RRID:Addgene_158791 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: ORF is at 258-2237.
The Addgene Quality Control sequencing results have found silent nucleotide changes in the depositor GSK3 beta sequence as well as an amino acid change. The verified sequence matches PBD Sequence ID: 4IQ6_A and encodes an isoform of human GSK3 beta that should function as WT, please review the Addgene QC sequence before use.
Proper citation: RRID:Addgene_15898 Copy
Species: Homo sapiens
Genetic Insert: GSK3 alpha
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2164470
Comments: ORF is at 1189-2640.
Proper citation: RRID:Addgene_15896 Copy
Species: Homo sapiens
Genetic Insert: trGluc
Vector Backbone Description: Backbone Size:8644; Vector Backbone:CSCW-IRES-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158959 Copy
Species: Homo sapiens
Genetic Insert: RAD51
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcdna3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30554948
Proper citation: RRID:Addgene_158953 Copy
Species: Homo sapiens
Genetic Insert: BACH1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:PcDNA_3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31257023
Proper citation: RRID:Addgene_159130 Copy
Species: Homo sapiens
Genetic Insert: Cdc42 (placental isoform)
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10087266
Comments: Dominant negative version of Cdc42 (G25K isoform)
Proper citation: RRID:Addgene_15907 Copy
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