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Species: Solanum lycopersicum
Genetic Insert: Nuclease (Cas9/sgRNA) + Donor + GVR
Vector Backbone Description: Backbone Marker:n/a; Vector Backbone:pLSLR; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26541286
Proper citation: RRID:Addgene_70019 Copy
Species: Homo sapiens
Genetic Insert: YTH N(6)-methyladenosine RNA binding protein 3
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEx-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24284625
Proper citation: RRID:Addgene_70088 Copy
Species: Homo sapiens
Genetic Insert: YTH N(6)-methyladenosine RNA binding protein 1
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEx-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24284625
Proper citation: RRID:Addgene_70087 Copy
Species: Homo sapiens
Genetic Insert: HOXB13
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:9573; Vector Backbone:pLX_TRC302; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26457646
Proper citation: RRID:Addgene_70089 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 10X-tRNA block
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pAG424-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29212664
Comments: The depositing lab notes that, despite the differences found in Addgene's QC sequence, this plasmid should work as advertised.
Proper citation: RRID:Addgene_70125 Copy
Species: Polyomavirus
Genetic Insert: TSPyV full genome, linearized in Large T-antigen
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20686659
Proper citation: RRID:Addgene_70033 Copy
Species: Homo sapiens
Genetic Insert: MKLP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22580824
Comments: Note that there are two silent mutations at position 2673 and 4203. The former is an artificial SalI site and the latter a polymorphism (I think). They also have Gly (GGG) artificially inserted between the starting methionine and the lysine 2.
Proper citation: RRID:Addgene_70154 Copy
Species: Synthetic
Genetic Insert: 5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4583; Vector Backbone:topo; Vector Types:Mouse Targeting, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26406378
Proper citation: RRID:Addgene_70155 Copy
Species: S. epidermidis
Genetic Insert: Cas10/Csm complex
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23935102
Proper citation: RRID:Addgene_70038 Copy
Species: Homo sapiens
Genetic Insert: MKLP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20451386
Comments: Note that there are two silent mutations at position 2682 and 4212. The former is an artificial SalI site and the latter a polymorphism (I think). They also have Gly (GGG) artificially inserted between the starting methionine and the lysine 2.
Proper citation: RRID:Addgene_70145 Copy
Species: Toxoplasma gondii
Genetic Insert: a selectable dihydrofolate reductase-thymidylate synthase marker
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2100; Vector Backbone:pUC19; Vector Types:Expression in Toxoplasma gondii; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26755159
Comments: Kevin Brown and Shaojun Long jointly built the plasmid in David Sibley lab, for CRISPR genetic operations.
Proper citation: RRID:Addgene_70147 Copy
Species: Synthetic
Genetic Insert: 5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:3952; Vector Backbone:topo; Vector Types:Mouse Targeting, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26406378
Proper citation: RRID:Addgene_70148 Copy
Species: Homo sapiens
Genetic Insert: ARAF
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70293 Copy
Species: Homo sapiens
Genetic Insert: ALK
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70292 Copy
Genetic Insert: 3xFLAG tag and aa 83-231 of Mnase
Vector Backbone Description: Vector Backbone:pFA6a-3HA-kanMX6; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26490019
Proper citation: RRID:Addgene_70231 Copy
Species: Synthetic
Genetic Insert: alpha Sarcin (N-terminal portion)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24481070
Proper citation: RRID:Addgene_70230 Copy
Genetic Insert: 3xFLAG tag and aa 83-231 of Mnase
Vector Backbone Description: Vector Backbone:pFA6a-3HA-Trp1; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26490019
Proper citation: RRID:Addgene_70233 Copy
Genetic Insert: 3xFLAG tag and aa 83-231 of Mnase
Vector Backbone Description: Vector Backbone:pFA6a-3HA-HIS3MX6; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26490019
Comments: Addgene's QC results indicate the selection marker may be a different version of HIS than found in the full sequence, but the depositor notes this does not affect function.
Proper citation: RRID:Addgene_70232 Copy
Genetic Insert: Short linker and aa 83-231 of Mnase
Vector Backbone Description: Vector Backbone:pFA6a-3HA-kanMX6; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26490019
Proper citation: RRID:Addgene_70234 Copy
Species: Other
Genetic Insert: H1-Tet-sgrna cassette
Vector Backbone Description: Backbone Marker:David Baltimore; Backbone Size:9940; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25732831
Proper citation: RRID:Addgene_70183 Copy
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