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Species: K. polysporus
Genetic Insert: DCR1(15-355)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4100; Vector Backbone:sumo-pRSFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21784247
Comments: The species of DCR1 is Kluyveromyces polysporus, which is also known as Vanderwaltozyma polyspora
Proper citation: RRID:Addgene_32026 Copy
Species: K. polysporus
Genetic Insert: DCR1(15-260)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4100; Vector Backbone:sumo-pRSFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21784247
Comments: The species of DCR1 is Kluyveromyces polysporus, which is also known as Vanderwaltozyma polyspora
Proper citation: RRID:Addgene_32029 Copy
Vector Backbone Description: Vector Backbone:pJW1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21368134
Proper citation: RRID:Addgene_32130 Copy
Genetic Insert: flpe
Vector Backbone Description: Vector Backbone:pMN210; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15563844
Comments: This is an expression vector for an enhanced Flp recombinase to excise FRT-flanked inserts in mycobacteria.
Proper citation: RRID:Addgene_32108 Copy
Species: Arabidopsis thaliana
Genetic Insert: A. thaliana tRNA ligase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5255; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20075163
Comments: We have found that this protein expresses best in the BL21 (DE3) RIPL cells from Stratagene. Standard BL21 (DE3) strains that lack tRNAs for rare codons do not work well (or at all).
Proper citation: RRID:Addgene_32242 Copy
Species: Mus musculus
Genetic Insert: shRNA against mouse HDAC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2854; Vector Backbone:pENTR/U6; Vector Types:RNAi, Gateway U6 ENTRY Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21565617
Proper citation: RRID:Addgene_32222 Copy
Genetic Insert: TALE Monomer NN
Vector Backbone Description: Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22222791
Comments: Please note that the uncut version of this plasmid runs differently than the other plasmids in this group. See the "Notes from Addgene" link above.
Proper citation: RRID:Addgene_32182 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 20XUAS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2609; Vector Backbone:pDONR 221 P1-P5r; Vector Types:Gateway MultiSite entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/12859 for additional plasmid map.
Proper citation: RRID:Addgene_32302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2667; Vector Backbone:pDONR 221 P4r-P3r; Vector Types:Gateway MultiSite entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/12862 for additional plasmid map.
Proper citation: RRID:Addgene_32309 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32412 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32416 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 L5-L4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32417 Copy
Genetic Insert: oPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Comments: Gene Therapy (2006) 13, 641–645. doi:10.1038/sj.gt.3302698; published online 15 December 2005
'Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression. Schambach A, Bohne J, Baum C, Hermann FG, Egerer L, von Laer D, Giroglou T. Gene Ther. 2006 Apr;13(7):641-5.'
Addgene Sanger sequencing confirms that all four ATGs in the oPRE have been mutated to TAGs
Proper citation: RRID:Addgene_32414 Copy
Genetic Insert: ColE1 origin replication
Vector Backbone Description: Vector Backbone:pMS2Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11707328
Comments: This is a mycobacterial shuttle vector with a lower copy number in E. coli.
Proper citation: RRID:Addgene_32360 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Schmidt-Dannert Lab; Backbone Size:4138; Vector Backbone:pBBRBB; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22033566
Proper citation: RRID:Addgene_32549 Copy
Species: Mus musculus
Genetic Insert: E1, ubiquitin-activating enzyme
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET-28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22012022
Proper citation: RRID:Addgene_32534 Copy
Vector Backbone Description: Backbone Size:12000; Vector Backbone:pCAMBIA1301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20388670
Proper citation: RRID:Addgene_32470 Copy
Vector Backbone Description: Backbone Size:12000; Vector Backbone:pCAMBIA1301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20388670
Proper citation: RRID:Addgene_32471 Copy
Species: Mus musculus
Genetic Insert: CAMKIIalpha promoter 1.3kb version
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P1-P4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32577 Copy
Species: Mus musculus
Genetic Insert: CAMKIIalpha promoter 0.4kb version
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32578 Copy
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