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Species: Synthetic
Genetic Insert: 3XHA-fCLIP-URA
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188922 Copy
Species: Synthetic
Genetic Insert: 3XHA-fCLIP-Kan
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188923 Copy
Species: Synthetic
Genetic Insert: 3XHA-eDHFR-NAT
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188926 Copy
Species: Synthetic
Genetic Insert: Prime editing pegRNA for SNCA-A53T
Vector Backbone Description: Backbone Marker:David Liu (Addgene plasmid # 132777); Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36069759
Proper citation: RRID:Addgene_181738 Copy
Species: Synthetic
Genetic Insert: 3XHA-HALO-Hygromycin
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188929 Copy
Species: Synthetic
Genetic Insert: URA-GAL1-fSNAP-3xHA
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188935 Copy
Species: Synthetic
Genetic Insert: URA-GAL1-fCLIP-3xHA
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188936 Copy
Species: Synthetic
Genetic Insert: URA-GAL1-fCLIP-GS
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188939 Copy
Species: Synthetic
Genetic Insert: GFPmut3
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Amp + Kan + Dap
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
Proper citation: RRID:Addgene_187381 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_187387 Copy
Species: Synthetic
Genetic Insert: IRES-creERT2-FNF
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This IRES-creERT2-FNF cassette was used in the Id1-IRES-creERT2 allele (http://www.informatics.jax.org/allele/MGI:4366863).
Compared to Bochkov and Palmenberg, 2006 (https://doi.org/10.2144/000112243), this EMCV IRES has a 1-bp deletion and an additional base of A ("7A"). The reason for these changes is unclear. These changes were in the electronic sequence file I received. The actual DNA sequence of the IRES was the same as the electronic sequence file. I incorporated this IRES into my plasmids without introducing additional changes. It was more recently tested in driving tdTomato expression (see https://www.addgene.org/185966/), and it was functional. I did not change those bases back and compare whether the expression level is then higher or lower. It may or may not be attenuated compared to the Bochkov and Palmenberg EMCV IRES.
Proper citation: RRID:Addgene_188230 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pkD4; Vector Types:Bacterial Expression; Bacterial Resistance:Amp + Kan + Dap
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
Proper citation: RRID:Addgene_187386 Copy
Species: Synthetic
Genetic Insert: 3XHA-fCLIP-Hygromycin
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188919 Copy
Species: Synthetic
Genetic Insert: 3XHA-fSNAP-NAT
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188916 Copy
Species: Synthetic
Genetic Insert: M13-TEV-C-P2A-TdTomato
Vector Backbone Description: Vector Backbone:pSyc 171 P2A vector; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650460
Proper citation: RRID:Addgene_92391 Copy
Species: Synthetic
Genetic Insert: TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650460
Proper citation: RRID:Addgene_92392 Copy
Species: Synthetic
Genetic Insert: Kar2-CFP-HDEL
Vector Backbone Description: Backbone Size:11752; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26362606
Comments: The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
Proper citation: RRID:Addgene_96989 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Size:2538; Vector Backbone:pBEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_97095 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Size:3191; Vector Backbone:pBEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_97098 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:6295; Vector Backbone:MSCVpuro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533508
Comments: Base vector obtained from Robert Hawley.
Proper citation: RRID:Addgene_97079 Copy
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