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Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7150; Vector Backbone:pRetroX-Tight-Pur; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26424696
Proper citation: RRID:Addgene_70185 Copy
Species: Synthetic
Genetic Insert: sfCherry(1-10)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26988139
Proper citation: RRID:Addgene_70222 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24481070
Proper citation: RRID:Addgene_70225 Copy
Genetic Insert: GFP(1-10)-VP64-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHRm; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26988139
Proper citation: RRID:Addgene_70228 Copy
Species: Synthetic
Genetic Insert: alpha Sarcin (N-terminal portion)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24481070
Proper citation: RRID:Addgene_70229 Copy
Species: Mus musculus
Genetic Insert: Eomes
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.); Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments:
The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_70271 Copy
Species: Mus musculus
Genetic Insert: GATA binding protein 3
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.) ; Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The coding sequence for murine Gata3 (together with parts of the 5' and 3' UTR) was inserted into the EcoRI site of the pLV-tetO vector (pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, Addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University)
Proper citation: RRID:Addgene_70270 Copy
Species: Mus musculus
Genetic Insert: Ets2
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.) ; Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The cDNA sequence of murine Ets2 was amplified from undifferentiated trophoblast stem cells and inserted into the pCR2.1 vector. From there it was excised by EcoRI digest and ligated into the pLV-tetO vector via the EcoRI site. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_70272 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:9080; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26375006
Comments: Subcloned Venus in place of Blast in lentiCas9-Blast.
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047 PubMed 25075903
Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan G, Zhang F, Orkin SH, Bauer DE. BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Nature. 2015 September 16; in press; doi:10.1038/nature15521.
Proper citation: RRID:Addgene_70267 Copy
Species: Homo sapiens
Genetic Insert: CCND2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70303 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: c969a, c972a, t1521c, c1524g, g2361a silent mutations not noted in Refseq
Proper citation: RRID:Addgene_70337 Copy
Species: Homo sapiens
Genetic Insert: E2F1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70329 Copy
Species: Homo sapiens
Genetic Insert: IRS2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: sequence codon optimized for cloning stability
Proper citation: RRID:Addgene_70396 Copy
Species: Homo sapiens
Genetic Insert: JUN
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70398 Copy
Species: Homo sapiens
Genetic Insert: CNKSR2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70313 Copy
Species: Homo sapiens
Genetic Insert: GRB2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70383 Copy
Species: Homo sapiens
Genetic Insert: FOS
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: c252t silent mutation not noted in Refseq
Proper citation: RRID:Addgene_70382 Copy
Species: Homo sapiens
Genetic Insert: PDPK1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70443 Copy
Species: Homo sapiens
Genetic Insert: PIK3R3
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70460 Copy
Species: Homo sapiens
Genetic Insert: RAF1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70497 Copy
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