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Species: Other
Genetic Insert: DEXsynth:mTb-ccdb+ RUBY
Vector Backbone Description: Vector Backbone:pICH75322; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_233321 Copy
Species: Other
Genetic Insert: DEXsynth:ccdb-3xHA + RUBY
Vector Backbone Description: Vector Backbone:pICH75322; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_233319 Copy
Species: Other
Genetic Insert: Ubi:ccdb + mcherry
Vector Backbone Description: Vector Backbone:pICH75322; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_233303 Copy
Species: Other
Genetic Insert: Ubi:ccdb + RUBY
Vector Backbone Description: Vector Backbone:pICH75322; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_233306 Copy
Species: Other
Genetic Insert: Anti-pTau nanobody (A2)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37961104
Comments: Please visit https://doi.org/10.1101/2023.10.24.563674 for bioRxiv preprint.
Proper citation: RRID:Addgene_233218 Copy
Species: Other
Genetic Insert: TurboID V5 T2A BFP
Vector Backbone Description: Vector Backbone:pLX303; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233257 Copy
Species: Other
Genetic Insert: BC(p1-10)
Vector Backbone Description: Vector Backbone:pscAAV-CAG-GFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains a CAG-driven EGFP
Proper citation: RRID:Addgene_231349 Copy
Species: Other
Genetic Insert: BC(p1-10)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains a CAG-driven EGFP
Proper citation: RRID:Addgene_231347 Copy
Species: Other
Genetic Insert: T7/T3-BC(p1-14)
Vector Backbone Description: Vector Backbone:pscAAV-CAG-GFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains a CAG-driven EGFP
Proper citation: RRID:Addgene_231346 Copy
Species: Other
Genetic Insert: PIP3 reporter
Vector Backbone Description: Vector Backbone:pLX303 mNG; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233261 Copy
Species: Other
Genetic Insert: T7-SP6-BC(p11-14)
Vector Backbone Description: Vector Backbone:pscAAV-CAG-GFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains a CAG-driven EGFP
Proper citation: RRID:Addgene_231350 Copy
Species: Other
Genetic Insert: BC(p1-10)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains a CMVe-SCP1-driven TagBFP2, with chimeric intron to increase expression
Proper citation: RRID:Addgene_231354 Copy
Species: Other
Genetic Insert: T7-SP6-BC(p11-14)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains an minBG-driven mRuby2, with chimeric intron to increase expression
Proper citation: RRID:Addgene_231353 Copy
Species: Other
Genetic Insert: T7-SP6-BC(p11-14)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains an mDLX-minBG-driven mRuby2, with chimeric intron to increase expression
Proper citation: RRID:Addgene_231352 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Proper citation: RRID:Addgene_231362 Copy
Species: Other
Genetic Insert: BC(p1-10)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains an mscRE4-minBG-driven EGFP, with chimeric intron to increase expression
Proper citation: RRID:Addgene_231361 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET-28b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24873830
Comments: Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis.
This plasmid was used to express a His-tagged Cas9 protein in E. coli and purify it by nickel-based affinity purification.The Cas9 protein and sgRNA were incubated at room temperature to form the Cas9 protein/sgRNA complex in vitro, which was then microinjected into zebrafish zygotes. See publication for more details.
Proper citation: RRID:Addgene_47327 Copy
Species: Other
Genetic Insert: Endophilin III
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_47410 Copy
Species: Other
Genetic Insert: Endophilin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2tk; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9341169
Proper citation: RRID:Addgene_47401 Copy
Species: Other
Genetic Insert: Intersectin SH3 domain E
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47400 Copy
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