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Species: Homo sapiens
Genetic Insert: RB1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70555 Copy
Species: Homo sapiens
Genetic Insert: RASSF3
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70541 Copy
Species: Homo sapiens
Genetic Insert: RASSF6
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_70547 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Backbone Size:9482; Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26375006
Comments: Cloned in Crimson Reporter in place of PURO reporter into lentiGuide-Puro.
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047 PubMed 25075903
Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan G, Zhang F, Orkin SH, Bauer DE. BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Nature. 2015 September 16; in press; doi:10.1038/nature15521.
Proper citation: RRID:Addgene_70683 Copy
Species: Homo sapiens
Genetic Insert: DDX3X
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26472758
Proper citation: RRID:Addgene_70648 Copy
Species: Homo sapiens
Genetic Insert: Fibrillarin
Vector Backbone Description: Vector Backbone:pTurboRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26472758
Proper citation: RRID:Addgene_70649 Copy
Genetic Insert: Citrine
Vector Backbone Description: Vector Backbone:pET21a(+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26430766
Proper citation: RRID:Addgene_70718 Copy
Species: Homo sapiens
Genetic Insert: hM3Dq-mCherry
Vector Backbone Description: Backbone Size:5142; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25609168
Proper citation: RRID:Addgene_70717 Copy
Genetic Insert: sgRNA against AAVS1
Vector Backbone Description: Vector Backbone:lentiCRISPR v1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26472758
Proper citation: RRID:Addgene_70661 Copy
Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus dCas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GGGCACGGGCAGCTTGCCGG. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.
Proper citation: RRID:Addgene_70703 Copy
Vector Backbone Description: Backbone Marker:Joachim Messing (Addgene plasmid # 50005); Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26524662
Comments: Use BsmBI sites to insert sgRNA spacer sequence.
The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA
Proper citation: RRID:Addgene_70709 Copy
Species: S. aureus
Genetic Insert: mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
Vector Backbone Description: Backbone Marker:Cepko lab (Addgene plasmid# 11179); Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26524662
Proper citation: RRID:Addgene_70708 Copy
Species: Homo sapiens
Genetic Insert: APEX1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET28c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26733580
Proper citation: RRID:Addgene_70757 Copy
Species: Ustilago maydis
Genetic Insert: U6 promoter
Vector Backbone Description: Backbone Marker:Weinzierl et al., 2002, Mol. Microbiol. 45; Vector Backbone:pNEBUC; Vector Types:self-replicating in Ustilago maydis, conferring carboxin resistance; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26365384
Proper citation: RRID:Addgene_70763 Copy
Species: Synthetic
Genetic Insert: Channelrhodopsin-2
Vector Backbone Description: Backbone Size:5601; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26443033
Proper citation: RRID:Addgene_70762 Copy
Species: Mus musculus
Genetic Insert: ATP Citrate Lyase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pEF6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24998913
Proper citation: RRID:Addgene_70765 Copy
Species: Mus musculus
Genetic Insert: inhibitor of DNA binding 2
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.); Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The cDNA sequence of murine Id2 was amplified and subcloned into the pCR2.1 vector. From there it was excised via HindIII and EcoRV and inserted into the pLV-tetO vector via the EcoRI site after Klenow treatment of both vector backbone and insert. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_70764 Copy
Species: Mus musculus
Genetic Insert: Crx-IRES-GFPd2
Vector Backbone Description: Backbone Marker:Addgene (Plasmid #14760); Backbone Size:4763; Vector Backbone:CAG-GFPd2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25155555
Proper citation: RRID:Addgene_73997 Copy
Species: Drosophila melanogaster
Genetic Insert: dU6-3:tRNA:gRNA:tRNA:gRNA
Vector Backbone Description: Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27595403
Comments: Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
Proper citation: RRID:Addgene_73914 Copy
Species: Homo sapiens
Genetic Insert: LC3A
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4970; Vector Backbone:pGex-4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24240096
Proper citation: RRID:Addgene_73946 Copy
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