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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pFP.R374 Resource Report Resource Website |
RRID:Addgene_138244 | araC | Other | Tetracycline | PMID:32251274 | Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:06:21 | 0 | ||
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pFP.E222 Resource Report Resource Website |
RRID:Addgene_138248 | araC | Other | Kanamycin | PMID:32251274 | Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:06:21 | 0 | ||
|
pET-TALE-mCherry-15b Resource Report Resource Website |
RRID:Addgene_138287 | 7-mer tTALE-mCherry | Other | Ampicillin | PMID:31748571 | Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Bacterial Expression, TALEN; Bacterial Resistance:Ampicillin | 2026-08-15 01:06:22 | 0 | ||
|
pCOM-CD63-4xM12-COM Resource Report Resource Website |
RRID:Addgene_138351 | COM-CD63-4xM12-COM fusion protein | Other | Kanamycin | PMID:33747370 | The insert sequence can be unstable during bacterial propagation. Restriction digests should be used to verify the full-length plasmid is present. | Backbone Marker:Addgene 62964; Backbone Size:3937; Vector Backbone:CD63-pEGFP C2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:06:22 | 0 | |
|
CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_138417 | BirA | Other | Ampicillin | PMID:32138752 | Backbone Marker:Takara Bio; Vector Backbone:pLVX-EF1a-zsGreen1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:06:22 | 1 | ||
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pBT120a17 Resource Report Resource Website |
RRID:Addgene_138510 | gIII-xLuxAB | Other | Ampicillin | PMID:32433547 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:06:24 | 0 | ||
|
pBT138b-pro1 Resource Report Resource Website |
RRID:Addgene_138513 | T7-RNAP | Other | Spectinomycin | PMID:32433547 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-15 01:06:24 | 0 | ||
|
pBT138b-proB Resource Report Resource Website |
RRID:Addgene_138515 | T7-RNAP | Other | Spectinomycin | PMID:32433547 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-15 01:06:24 | 0 | ||
|
pBT138b-proD Resource Report Resource Website |
RRID:Addgene_138516 | T7-RNAP | Other | Spectinomycin | PMID:32433547 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-15 01:06:24 | 0 | ||
|
pBT100.164 Resource Report Resource Website |
RRID:Addgene_138522 | ecTadA(7.10)-inteinN | Other | Kanamycin | PMID:32433547 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:06:24 | 0 | ||
|
pBT29 Resource Report Resource Website |
RRID:Addgene_138521 | phage genes | Other | Kanamycin | PMID:32433547 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:06:24 | 0 | ||
|
p667-UBC-GFP-V5-miniTurbo_IDG-K Resource Report Resource Website |
RRID:Addgene_135240 | GFP-V5-miniTurbo | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:51 | 0 | ||
|
p667-UBC-GFP-V5-TurboID_IDG-K Resource Report Resource Website |
RRID:Addgene_135241 | GFP-V5-TurboID | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:51 | 0 | ||
|
p663-UBC-TurboID-V5-dsRed_IDG-K Resource Report Resource Website |
RRID:Addgene_135261 | TurboID-V5-dsRed | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:52 | 0 | ||
|
p663-UBC-V5-dsRed_IDG-K Resource Report Resource Website |
RRID:Addgene_135262 | V5-dsRed | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:52 | 0 | ||
|
p663-UBC-miniTurbo-V5-eGFP_IDG-K Resource Report Resource Website |
RRID:Addgene_135263 | miniTurbo-V5-eGFP | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:52 | 0 | ||
|
pHAGE-CMV-Flag-hcRed_IDG-K Resource Report Resource Website |
RRID:Addgene_135274 | Flag-hcRed | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Size:9763; Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:05:52 | 0 | ||
|
3TERM_35S Resource Report Resource Website |
RRID:Addgene_136114 | 3TERM_35S (CaMV) | Other | Chloramphenicol | PMID:32163700 | Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint. | Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | BsaI/ SapI domesticated | 2026-08-15 01:06:02 | 0 |
|
3TERM_Nos Resource Report Resource Website |
RRID:Addgene_136115 | 3TERM_Nos (A. tumefaciens) | Other | Chloramphenicol | PMID:32163700 | Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint. | Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | BsaI/ SapI domesticated | 2026-08-15 01:06:02 | 0 |
|
pRDA_174 Resource Report Resource Website 10+ mentions |
RRID:Addgene_136476 | EnAsCas12a | Other | Ampicillin | PMID:32661438 | Vector Backbone:pLKO; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:06:06 | 13 |
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