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Species: Synthetic
Genetic Insert: pAce-Kv2.1 proximal restriction sequence
Vector Backbone Description: Vector Backbone:pAAV-Synapsin; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36378956
Proper citation: RRID:Addgene_195523 Copy
Species: Synthetic
Genetic Insert: pAceR-Kv2.1 proximal restriction sequence
Vector Backbone Description: Vector Backbone:pAAV-Synapsin; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36378956
Proper citation: RRID:Addgene_195525 Copy
Species: Synthetic
Genetic Insert: pAceR
Vector Backbone Description: Vector Backbone:pAAV-Synapsin; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36378956
Proper citation: RRID:Addgene_195521 Copy
Species: Synthetic
Genetic Insert: mScarlet-i
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985281
Proper citation: RRID:Addgene_190614 Copy
Species: Synthetic
Genetic Insert: mScarlet-I
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.26.437257v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185943 Copy
Species: Synthetic
Genetic Insert: Ig kappa-FRISZ-PDGFR TM
Vector Backbone Description: Backbone Size:4280; Vector Backbone:pAAV-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36857451
Comments: The N-terminal Ig kappa leader sequence is for targeting the synthesized peptide to the secretory pathway. The C-terminal PDGFR transmembrane domain is for anchoring the protein to the mammalian cell surface. AAV derived from this plasmid has been successfully used for wide-field in vivo imaging. We deposit another plasmid, pAAV-hSyn-FRISZ-GPI (Addgene # 177264, not yet tested in vivo), which uses a CD59 leader sequence for secretory pathway targeting and a CD59 GPI for anchoring. We plan to systematically compare various exporting and anchoring strategies.
Please visit https://www.biorxiv.org/content/10.1101/2022.06.02.494512v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176886 Copy
Species: Synthetic
Genetic Insert: mEos3.2
Vector Backbone Description: Backbone Marker:Thermo; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35903559
Proper citation: RRID:Addgene_189719 Copy
Species: Synthetic
Genetic Insert: THP4
Vector Backbone Description: Vector Backbone:pBR322-ROP; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35799063
Proper citation: RRID:Addgene_190671 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:5055; Vector Backbone:BASIC_SEVA_15a.10; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178913 Copy
Species: Synthetic
Genetic Insert: KOC207
Vector Backbone Description: Vector Backbone:pGWT35S; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29661017
Proper citation: RRID:Addgene_182730 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:4422; Vector Backbone:TRE3g; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36198803
Proper citation: RRID:Addgene_192064 Copy
Species: Synthetic
Genetic Insert: zsGreen
Vector Backbone Description: Backbone Marker:Sanford Simon; Backbone Size:8230; Vector Backbone:pLVX-Che-hi3; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34812139
Proper citation: RRID:Addgene_153320 Copy
Species: Synthetic
Genetic Insert: L-sDarken
Vector Backbone Description: Backbone Size:4368; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36473867
Comments: Please visit https://doi.org/10.1101/2022.03.10.483799 for bioRxiv preprint.
Proper citation: RRID:Addgene_184798 Copy
Species: Synthetic
Genetic Insert: ymNeonGreen
Vector Backbone Description: Backbone Size:700; Vector Backbone:pCEV-G2-KM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_193959 Copy
Species: Synthetic
Genetic Insert: miRFP718nano
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36456785
Comments: Modifications to backbone: Several amino acids between His-tag and miRFP718nano were removed
Proper citation: RRID:Addgene_195743 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:3601; Vector Backbone:Modified pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31255486
Proper citation: RRID:Addgene_195558 Copy
Species: Synthetic
Genetic Insert: TadA8.20
Vector Backbone Description: Backbone Size:5201; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593412
Proper citation: RRID:Addgene_194702 Copy
Species: Synthetic
Genetic Insert: ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
Vector Backbone Description: Vector Backbone:pSIN; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31592660
Proper citation: RRID:Addgene_192636 Copy
Species: Synthetic
Genetic Insert: tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Vector Backbone Description: Backbone Size:6288; Vector Backbone:pSin-EF1a; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31592660
Proper citation: RRID:Addgene_176311 Copy
Species: Synthetic
Genetic Insert: Tornado-Pepper-RhoBAST
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37391423
Comments: Cloned using pAV-U6+27-Tornado-Broccoli(#124360) . Please visit https://www.biorxiv.org/content/10.1101/2022.10.24.513449v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_196343 Copy
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