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Species: Synthetic
Genetic Insert: Tornado-RhoBAST
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33574610
Comments: Cloned using pAV-U6+27-Tornado-Broccoli(#124360)
Proper citation: RRID:Addgene_196341 Copy
Species: Synthetic
Genetic Insert: eHcKCR1 3.0-oScarlet-Kv2.1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5623; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36669484
Proper citation: RRID:Addgene_195198 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:H. Oguro, unpublished; Vector Backbone:PB-EF1a-PuroR-EGFP; Vector Types:Mammalian Expression, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36459969
Comments: This plasmid was generated by inserting the PG13-mCherry sequence (Addgene 195291) into the PB-EF1a-PuroR-EGFP plasmid (H. Oguro, unpublished), and contains the piggyBac 3'-inverted terminal repeat (PB 3'ITR), 5'-HS4 chicken b-globin (cHS4) insulator, PG13-mCherry reporter, woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine growth hormone polyadenylation signal (bGHpA), EF1a promoter, puromycin registrant gene, P2A peptide, EGFP, bGHpA, cHS4 insulator, and PB 5'ITR, in this order.
Proper citation: RRID:Addgene_195292 Copy
Species: Synthetic
Genetic Insert: NLS-mCherry
Vector Backbone Description: Vector Backbone:PLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36450983
Proper citation: RRID:Addgene_195332 Copy
Species: Synthetic
Genetic Insert: mAzurite-16xRhoBAST
Vector Backbone Description: Vector Backbone:mAzurite-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37391423
Comments: Cloned using mAzurite-C1 (#54583). Please visit https://www.biorxiv.org/content/10.1101/2022.10.24.513449v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_196347 Copy
Species: Synthetic
Genetic Insert: Tornado-biRhoBAST
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36658339
Comments: Cloned using pAV-U6+27-Tornado-Broccoli(#124360) . Please visit https://www.biorxiv.org/content/10.1101/2021.11.02.466936v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_196344 Copy
Species: Synthetic
Genetic Insert: mEGFP-8xbiRhoBAST
Vector Backbone Description: Vector Backbone:mAzurite-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36658339
Comments: Cloned using mAzurite-C1 (#54583) . Please visit https://www.biorxiv.org/content/10.1101/2021.11.02.466936v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_196351 Copy
Species: Synthetic
Genetic Insert: mAzurite-8xbiRhoBAST-24xMS2
Vector Backbone Description: Vector Backbone:mAzurite-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36658339
Comments: Cloned using mAzurite-C1 (#54583) . Please visit https://www.biorxiv.org/content/10.1101/2021.11.02.466936v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_196352 Copy
Species: Synthetic
Genetic Insert: GLPLight1
Vector Backbone Description: Backbone Marker:Viral Vector Facility - University of Zurich; Backbone Size:4715; Vector Backbone:pAAV.CAG; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37265064
Comments: Please visit https://doi.org/10.1101/2023.02.14.528498 for bioRxiv preprint.
Proper citation: RRID:Addgene_187467 Copy
Species: Synthetic
Genetic Insert: 92-1 Op (Part 2)
Vector Backbone Description: Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194487 Copy
Species: Synthetic
Genetic Insert: gfasPurple
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34767345
Comments: Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180549 Copy
Species: Synthetic
Genetic Insert: PKIαmut-P2A-mRuby3
Vector Backbone Description: Vector Backbone:pAAV w CAG promotor, FLEX cassette and WPRE; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36352228
Proper citation: RRID:Addgene_196678 Copy
Species: Synthetic
Genetic Insert: tAKARα
Vector Backbone Description: Vector Backbone:pAAV w SynP, DIO cassette and WPRE; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36352228
Proper citation: RRID:Addgene_196675 Copy
Species: Synthetic
Genetic Insert: ABE8e-NG C-term
Vector Backbone Description: Backbone Size:4735; Vector Backbone:modified pX601; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36797483
Proper citation: RRID:Addgene_194652 Copy
Species: Synthetic
Genetic Insert: Enhanced Green Fluorescent Protein
Vector Backbone Description: Vector Backbone:E1b-GFP-Tol2; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36167150
Comments: Eye lens-specific cryaa:mCherry cassette serves as transgenesis marker
Proper citation: RRID:Addgene_194516 Copy
Species: Synthetic
Genetic Insert: Lck-smFLAG
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3406; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, Astrocyte-selective; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37973910
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.21.529451v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_196414 Copy
Species: Synthetic
Genetic Insert: Lck-smV5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3406; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, Astrocyte-selective; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37973910
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.21.529451v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_196416 Copy
Species: Synthetic
Genetic Insert: Hsp2Cas9
Vector Backbone Description: Vector Backbone:AAV2 ITR vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36733251
Proper citation: RRID:Addgene_192126 Copy
Species: Synthetic
Genetic Insert: Cj-SgRNA scaffold
Vector Backbone Description: Vector Backbone:pUC57 vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36733251
Proper citation: RRID:Addgene_192128 Copy
Species: Synthetic
Genetic Insert: Booster-PKA
Vector Backbone Description: Vector Backbone:pCSIIpuro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32101394
Comments: The linker region between mKate2 and mKO2 is extremely repeat rich and difficult to validate using Sanger or short read NGS. Requesting scientists should validate expected length using restriction digests in addition to sequencing methods and functionality prior to experimental use.
Proper citation: RRID:Addgene_175811 Copy
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