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Species: Homo sapiens
Genetic Insert: GNB2 5'-UTR
Vector Backbone Description: Vector Backbone:pFL-SV40; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30373933
Comments: Note: Plasmid contains a GCC deletion and a T to C change in the GNB2 5'UTR. These changes are not known to affect plasmid function.
Proper citation: RRID:Addgene_115355 Copy
Species: Homo sapiens
Genetic Insert: TAK1 5'-UTR
Vector Backbone Description: Vector Backbone:pFL-SV40; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30373933
Comments: Note: Plasmid contains an A to G nucleotide change within the TAK1 5'UTR. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_115356 Copy
Species: Homo sapiens
Genetic Insert: shMBNL3-0455
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115463 Copy
Species: Homo sapiens
Genetic Insert: shMBNL3-9465
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115464 Copy
Species: Homo sapiens
Genetic Insert: shMBNL2-3712
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115461 Copy
Species: Homo sapiens
Genetic Insert: shMBNL2-1082
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115462 Copy
Species: Homo sapiens
Genetic Insert: shSNAI1
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115467 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Size:5353; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10797013
Proper citation: RRID:Addgene_11558 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28276439
Proper citation: RRID:Addgene_115617 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28276439
Proper citation: RRID:Addgene_115618 Copy
Species: Homo sapiens
Genetic Insert: TSPAN12
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5407; Vector Backbone:pcDNA3.3 TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28658627
Comments: N-terminal HA tag has no substantial effect in TOPFlash reporter assays induced with Norrin, compared to untagged control
Proper citation: RRID:Addgene_115784 Copy
Species: Homo sapiens
Genetic Insert: scFv-sfGFP-DNMT3A1
Vector Backbone Description: Backbone Marker:Stephen Elledge; Backbone Size:11791; Vector Backbone:Pinducer 20; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30097071
Proper citation: RRID:Addgene_115780 Copy
Species: Homo sapiens
Genetic Insert: FZD4
Vector Backbone Description: Backbone Size:5407; Vector Backbone:pcDNA3.3 TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28658627
Comments: N-terminal V5 tag has no substantial effect in TOPFlash reporter assays induced with Norrin, compared to untagged control. TOPFlash reporter assay can be inhibited by transfection of too much of this plasmid (see Lai et al., 2017 for detailed description of transfection conditions).
Proper citation: RRID:Addgene_115787 Copy
Species: Homo sapiens
Genetic Insert: BMP2
Vector Backbone Description: Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.
Proper citation: RRID:Addgene_115771 Copy
Species: Homo sapiens
Genetic Insert: NGRN
Vector Backbone Description: Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.
Proper citation: RRID:Addgene_115773 Copy
Species: Homo sapiens
Genetic Insert: Androgen Receptor
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8175737
Comments: Insert encodes 25 glutamines in the polymorphic region of the androgen receptor. Mutations are based on the numbering from the associated publication.
Proper citation: RRID:Addgene_115778 Copy
Species: Homo sapiens
Genetic Insert: CBLN3
Vector Backbone Description: Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.
Proper citation: RRID:Addgene_115768 Copy
Species: Homo sapiens
Genetic Insert: CBLN4
Vector Backbone Description: Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.
Proper citation: RRID:Addgene_115769 Copy
Species: Homo sapiens
Genetic Insert: CBLN1
Vector Backbone Description: Backbone Size:111; Vector Backbone:pCMV6-XL4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon Optimized, FLAG-tag is followed by a NotI site and hIgG-tag is preceeded by a XbaI site. The reverse sequencing primer will sequence the CBLN1 insert only and not the hIgG-tag.
Proper citation: RRID:Addgene_115765 Copy
Species: Homo sapiens
Genetic Insert: SETDB1 shRNA
Vector Backbone Description: Vector Backbone:pAPM-D4-mir30; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30297740
Comments: PuroR . Please visit https://www.biorxiv.org/content/early/2018/04/03/293001 for bioRxiv preprint.
Proper citation: RRID:Addgene_115860 Copy
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