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Species: Synthetic
Genetic Insert: pET28 MBP-HNLYGHS-GST
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29038472
Proper citation: RRID:Addgene_98812 Copy
Species: Synthetic
Genetic Insert: PROPS
Vector Backbone Description: Backbone Marker:Bio Bricks - iGem parts combined by Ben Dodd; Backbone Size:2384; Vector Backbone:BB118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28808010
Proper citation: RRID:Addgene_98919 Copy
Species: Synthetic
Genetic Insert: GCaMP6f tethered to mRuby
Vector Backbone Description: Backbone Marker:Bio Bricks - iGem parts combined by Ben Dodd; Backbone Size:2384; Vector Backbone:BB118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28808010
Proper citation: RRID:Addgene_98920 Copy
Species: Synthetic
Genetic Insert: Ruby2-smFP-Flag
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Comments: Penn Vector Core PV3509
Proper citation: RRID:Addgene_98925 Copy
Species: Synthetic
Genetic Insert: GFP-smFP-myc
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Comments: Penn Vector Core PV3661
Proper citation: RRID:Addgene_98927 Copy
Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2723. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98929 Copy
Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2914. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98930 Copy
Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2724. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98931 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Mathupala, S.P.; Backbone Size:6059; Vector Backbone:pLXIN2; Vector Types:Mammalian Expression, Retroviral, Bicistronic retroviral expression vector; Bacterial Resistance:Ampicillin
Comments: When transfected into amphotropic (PA317) or ecotropic (PE501) mammalian packaging cell lines, the plasmid pLXIN2 will generate live retrovirus. Thus, the procedures will require handling at BSL2 level, when the plasmid is used to generate recombinant retrovirus for transduction.
Please note the Kozak sequence is missing a nucleotide; however, this has no functional consequence for expression.
Proper citation: RRID:Addgene_99204 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Dr. Dusty Miller, Fred Hutchinson Cancer Center, Seattle, WA; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: When transfected into packaging cell lines PE501 (ecotropic) or PA317 (amphotropic) the plasmid will generate live retrovirus. Thus, under these conditions BSL2 safety will need to be observed.
Proper citation: RRID:Addgene_99207 Copy
Species: Synthetic
Genetic Insert: Cas9-RFC5
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR221 (2184742_AE175); Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34898428
Proper citation: RRID:Addgene_183200 Copy
Species: Synthetic
Genetic Insert: sgRNA_6E
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176870 Copy
Species: Synthetic
Genetic Insert: sgRNA_4
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176867 Copy
Species: Synthetic
Genetic Insert: sgRNA_4E
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176868 Copy
Species: Synthetic
Genetic Insert: Cas9-POLD3
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR221 (2184742_AE175); Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34898428
Proper citation: RRID:Addgene_183197 Copy
Species: Synthetic
Genetic Insert: Citrine
Vector Backbone Description: Vector Backbone:pGWT35S; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28894649
Proper citation: RRID:Addgene_182581 Copy
Species: Synthetic
Genetic Insert: TagRFP-T with palmitoylation signal at N-terminus
Vector Backbone Description: Backbone Size:7818; Vector Backbone:pHAGE-Ubc; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078295
Proper citation: RRID:Addgene_178656 Copy
Species: Synthetic
Genetic Insert: frSkylan-S
Vector Backbone Description: Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34495704
Proper citation: RRID:Addgene_182209 Copy
Species: Synthetic
Genetic Insert: KON206
Vector Backbone Description: Vector Backbone:pGWT35S; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29661017
Proper citation: RRID:Addgene_182729 Copy
Species: Synthetic
Genetic Insert: KOC169
Vector Backbone Description: Vector Backbone:pGWT35S; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29661017
Proper citation: RRID:Addgene_182728 Copy
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