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Species: Danio rerio
Genetic Insert: mfn2 gRNA
Vector Backbone Description: Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32788232
Proper citation: RRID:Addgene_121994 Copy
Species: Danio rerio
Genetic Insert: opa1 gRNA
Vector Backbone Description: Backbone Marker:Tol2 kit; Backbone Size:4000; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32788232
Proper citation: RRID:Addgene_121995 Copy
Vector Backbone Description: Backbone Marker:Esteban Martínez-García and Víctor de Lorenzo; Backbone Size:3168; Vector Backbone:pEMG; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122086 Copy
Species: Homo sapiens
Genetic Insert: Brg1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Proper citation: RRID:Addgene_122115 Copy
Species: Homo sapiens
Genetic Insert: Brg1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Proper citation: RRID:Addgene_122117 Copy
Species: Homo sapiens
Genetic Insert: His6 MBP TEV HDAC8_374
Vector Backbone Description: Backbone Marker:Scott Gradia; Backbone Size:6465; Vector Backbone:pET His6 MBP TEV LIC cloning vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28846375
Proper citation: RRID:Addgene_122174 Copy
Species: Aequorea victoria
Genetic Insert: RS-GFP
Vector Backbone Description: Backbone Marker:prof. Pascal Ratet (French National Centre for Scientific Research); Backbone Size:12400; Vector Backbone:pCP60 (sequence not available in databases) - derivative of pBIN19; Vector Types:Plant Expression, binary vector for Escherichia coli and Agrobacterium tumefaciens-mediated plant transformation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19386122
Proper citation: RRID:Addgene_122173 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122253 Copy
Species: Caenorhabditis elegans
Genetic Insert: mtagBFP2
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122255 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122254 Copy
Species: Synthetic
Genetic Insert: gRNAwcaF159
Vector Backbone Description: Vector Backbone:pBbE2k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30177768
Proper citation: RRID:Addgene_122259 Copy
Species: Homo sapiens
Genetic Insert: NPM-ALK fusion protein
Vector Backbone Description: Backbone Size:5289; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32193476
Proper citation: RRID:Addgene_122462 Copy
Genetic Insert: Strep-tagged 5xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122492 Copy
Genetic Insert: Strep-tagged 10xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122497 Copy
Genetic Insert: Strep-tagged 9xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122496 Copy
Genetic Insert: Strep-tagged 12xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122499 Copy
Species: Homo sapiens
Genetic Insert: tUI
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122661 Copy
Species: Homo sapiens
Genetic Insert: tISR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122664 Copy
Species: Homo sapiens
Genetic Insert: tIVR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122663 Copy
Species: Homo sapiens
Genetic Insert: ferritin heavy chain 1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_122652 Copy
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