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Species: Synthetic
Genetic Insert: Promoter SPLc25 and RBSc249
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171905 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is an amino terminal HIS tag, TEV protease cleavage site, then human beta catenin domains R1C, subcloned in BamHI and Xho 1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17200 Copy
Species: Xenopus laevis
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is an amino terminal HIS tag, then beta catenin domains R10C, subcloned into BamH1 site.
Reference: Made by Ken-Ichi Takemaru.
Contains ARM domains 8-10 and C-terminal end of protein (starts with aa519)
Proper citation: RRID:Addgene_17202 Copy
Species: Homo sapiens
Genetic Insert: E3 ubiquitin-protein ligase RNF216
Vector Backbone Description: Backbone Size:5642; Vector Backbone:pOPINB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34998453
Proper citation: RRID:Addgene_171922 Copy
Species: Synthetic
Genetic Insert: RNA-based (p)ppGpp sensor
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5267; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34487413
Comments: Addgene QC identified a C66Y change in the lacI coding sequence that may impact the leakiness of expression of the (p)ppGpp sensor and NirFP insert.
Proper citation: RRID:Addgene_171921 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc45 and RBSc21
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171887 Copy
Species: Synthetic
Genetic Insert: RNA-based (p)ppGpp sensor
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5213; Vector Backbone:pET-28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34487413
Proper citation: RRID:Addgene_171920 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc25 and RBSc21
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171886 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc3 and RBSc21
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171884 Copy
Species: Synthetic
Genetic Insert: Cys-DogCatcher
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171929 Copy
Species: llama
Genetic Insert: VHH_H3
Vector Backbone Description: Backbone Size:5564; Vector Backbone:pOPINO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34552091
Proper citation: RRID:Addgene_171927 Copy
Species: llama
Genetic Insert: VHH_C5
Vector Backbone Description: Backbone Size:5564; Vector Backbone:pOPINO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34552091
Proper citation: RRID:Addgene_171925 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc17 and RBSc123
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171912 Copy
Vector Backbone Description: Vector Backbone:pStA212; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171878 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc17 and RBSc48
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171910 Copy
Species: Homo sapiens
Genetic Insert: Tandem repeats Hybrid binding domain of Rnase H1
Vector Backbone Description: Vector Backbone:PGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33597247
Proper citation: RRID:Addgene_171870 Copy
Species: Homo sapiens
Genetic Insert: Chibby
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The N terminal half (amino acids 1-63) of human Chibby was amplified by PCR and subcloned into EcoR1 and Xho1 sites of pGEX-4T-1. A TEV protease site was included in the 5' primer.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_17196 Copy
Species: Homo sapiens
Genetic Insert: Chibby
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The C terminal half (amino acids 64-126) of human Chibby was amplified by PCR and subcloned into EcoR1 and Xho1 sites of pGEX-4T-1. A TEV protease site was included in the 5' primer. Insert contains 3'UTR of Cby.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_17197 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is full length human beta catenin cDNA with a TEV protease site at the amino terminus following the HIS tag. Cloned into BamH1 and Xho1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17198 Copy
Species: Danio rerio
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is full length zebrafish beta catenin cDNA with a TEV protease site at the amino terminus following the HIS tag. Cloned into Sal1 and Not1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17199 Copy
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