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Species: Homo sapiens
Genetic Insert: TDP43
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30500534
Comments: Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.
Proper citation: RRID:Addgene_122669 Copy
Species: Homo sapiens
Genetic Insert: tUI
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122661 Copy
Species: Homo sapiens
Genetic Insert: tISR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122664 Copy
Species: Homo sapiens
Genetic Insert: tIVR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122663 Copy
Species: Homo sapiens
Genetic Insert: ferritin heavy chain 1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_122652 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens forkhead box P2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5269; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30887622
Proper citation: RRID:Addgene_122647 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens forkhead box P2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5269; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30887622
Proper citation: RRID:Addgene_122649 Copy
Species: Homo sapiens
Genetic Insert: CXCR4 siRNA
Vector Backbone Description: Backbone Size:7139; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15882617
Comments: CXCR4-siRNA-1 sequence: 5'-TGGAGGGGATCAGTATATACA-3'
Proper citation: RRID:Addgene_12271 Copy
Species: Homo sapiens
Genetic Insert: Pyruvate kinase M2 isoform
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911680
Proper citation: RRID:Addgene_122691 Copy
Species: Homo sapiens
Genetic Insert: Pyruvate kinase M2 isoform
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911680
Proper citation: RRID:Addgene_122690 Copy
Species: Homo sapiens
Genetic Insert: Pyruvate kinase M2 isoform
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911680
Proper citation: RRID:Addgene_122693 Copy
Species: Homo sapiens
Genetic Insert: Pyruvate kinase M2 isoform
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911680
Proper citation: RRID:Addgene_122692 Copy
Species: Homo sapiens
Genetic Insert: irisin (ectodomain of FNDC5)
Vector Backbone Description: Backbone Marker:novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25749243
Proper citation: RRID:Addgene_122612 Copy
Species: Homo sapiens
Genetic Insert: MyD88
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Addgene's sequencing results indicate the MyD88 insert starts at aa15 when compared to GenBank reference sequence NP_002459.2 (MyD88 isoform 2). When numbered according to this reference sequence, the MyD88 mutation is P213H
Proper citation: RRID:Addgene_12288 Copy
Species: Homo sapiens
Genetic Insert: H-Ras G12V E37G
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15324700
Proper citation: RRID:Addgene_12277 Copy
Species: Homo sapiens
Genetic Insert: H-Ras G12V Y40C
Vector Backbone Description: Backbone Size:5150; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15324700
Comments: A BamHI-SalI fragment from the pWZL Blast ras mutants were individually cloned into the pBABE puro vector directionally.
Proper citation: RRID:Addgene_12276 Copy
Species: Homo sapiens
Genetic Insert: H-Ras G12V Y40C
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15324700
Proper citation: RRID:Addgene_12279 Copy
Species: Homo sapiens
Genetic Insert: H-Ras G12V T35S
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15324700
Proper citation: RRID:Addgene_12278 Copy
Species: Homo sapiens
Genetic Insert: CXCR4 siRNA
Vector Backbone Description: Backbone Size:7139; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15882617
Comments: CXCR4-siRNA-2 sequence: 5'-GTTTTCACTCCAGCTAACACA-3'
Proper citation: RRID:Addgene_12272 Copy
Species: Homo sapiens
Genetic Insert: human NPHP1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34085951
Proper citation: RRID:Addgene_122872 Copy
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