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Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:Bluescript; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16400644
Proper citation: RRID:Addgene_21548 Copy
Species: Mus musculus
Genetic Insert: Stra8 promoter
Vector Backbone Description: Backbone Size:7048; Vector Backbone:pLLU2G; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18719224
Comments: This is a 3rd generation lentiviral plasmid.
Proper citation: RRID:Addgene_21619 Copy
Species: Mus musculus
Genetic Insert: Bmi-1
Vector Backbone Description: Backbone Marker:D. Baltimore; Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371338
Comments: Bmi-1 cDNA cloned into pIRES-eGFP first. The Bmi1-ires-eGFP was then cut out and cloned into FUGW.
Proper citation: RRID:Addgene_21577 Copy
Species: Homo sapiens
Genetic Insert: ALIX
Vector Backbone Description: Backbone Size:4700; Vector Backbone:mCherry; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18948538
Proper citation: RRID:Addgene_21504 Copy
Species: Homo sapiens
Genetic Insert: MKK6
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9712898
Comments: M2-FLAG,
Lysine mutant, inactive
Proper citation: RRID:Addgene_21583 Copy
Vector Backbone Description: Backbone Marker:Luk van Parijs; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: hUbC promoter was inserted upstream of EGFP.
There are minor sequence discrepancies with depositor's sequence. Depositor is aware of the mismatches and they are acceptable.
Proper citation: RRID:Addgene_21620 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 2
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11784851
Proper citation: RRID:Addgene_21586 Copy
Species: evolved fluorescent protein
Genetic Insert: mNectarine fluorescent proteins
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad/His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19494110
Proper citation: RRID:Addgene_21717 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 2 alpha
Vector Backbone Description: Backbone Size:5188; Vector Backbone:pBabe-Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17526729
Proper citation: RRID:Addgene_21676 Copy
Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pMSCV PIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation.
Constructs for Soft-agar assay
Proper citation: RRID:Addgene_21659 Copy
Species: Homo sapiens
Genetic Insert: Dicer 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Mut means that all microRNA binding sites for an indicated microRNA have been mutated.
Proper citation: RRID:Addgene_21653 Copy
Species: Homo sapiens
Genetic Insert: E2F-1
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4959; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1638635
Proper citation: RRID:Addgene_21668 Copy
Species: Homo sapiens
Genetic Insert: Orai1
Vector Backbone Description: Backbone Size:7480; Vector Backbone:MO70; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17293345
Comments: See author's map for image of empty MO70 vector (MO70 contains IRES-GFP after the multiple cloning site).
Proper citation: RRID:Addgene_21662 Copy
Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation
Proper citation: RRID:Addgene_21639 Copy
Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18337722
Proper citation: RRID:Addgene_21753 Copy
Species: M. magnetotacticum
Genetic Insert: magA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11250081
Comments: The sequence inserted into the pCR2.1 vector is larger than just the magA gene. The magA gene is preceded by 373 bp of genomic DNA and followed by 30 bp of genomic DNA. The gene contains 2 amino acid substitutions - S94L and P390S.
The cloned gene was used primarily as a probe. Also see Zurkiya et al. Magn Reson Med. 2008 Jun;59(6):1225-31.
Proper citation: RRID:Addgene_21751 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D2 long UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Proper citation: RRID:Addgene_21645 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D2 short UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation.
Proper citation: RRID:Addgene_21644 Copy
Species: Homo sapiens
Genetic Insert: hOCT4 - 2A - hSOX2 - 2A - hKLF4
Vector Backbone Description: Backbone Size:9692; Vector Backbone:pDL171 (pWPI with deletion of 1409 bp XhoI [EMCV IRES GFP] frag); Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19415770
Comments: Plasmid constructed by Kevin Pawlik
Proper citation: RRID:Addgene_21627 Copy
Species: Homo sapiens
Genetic Insert: SRF
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pEntr3c; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21415370
Proper citation: RRID:Addgene_32971 Copy
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