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Species: Synthetic
Genetic Insert: GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35254056
Proper citation: RRID:Addgene_183400 Copy
Species: Synthetic
Genetic Insert: LgBiT-CaM
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35254056
Proper citation: RRID:Addgene_183401 Copy
Species: Synthetic
Genetic Insert: V5-APEX2-NES
Vector Backbone Description: Vector Backbone:pAAV-VTKD3; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178697 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKS4; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178730 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKS5; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178731 Copy
Species: Synthetic
Genetic Insert: V5-APEX2-NES
Vector Backbone Description: Vector Backbone:pAAV-VTKD2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178696 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKD4; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178727 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKD5; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178728 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKD6; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178729 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKD6; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178723 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKS4; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178724 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKD5; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178722 Copy
Species: Synthetic
Genetic Insert: EBFP2-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184461 Copy
Species: Synthetic
Genetic Insert: TagRFP-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184447 Copy
Species: Synthetic
Genetic Insert: TetOn3G
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6370; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control)
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179887 Copy
Species: Synthetic
Genetic Insert: LiLac fluorescent sensor of lactate
Vector Backbone Description: Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35614105
Comments: mTurquoise2 sequences are originally from Goedhart, J. et al. Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun 3, 751 (2012) and were obtained by PCR from the plasmid encoding the Lck-cpmTq2-Calcium-lifetime-sensor (Addgene plasmid #129627) (A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. van der Linden FH, Mahlandt EK, Arts JJG, Beumer J, Puschhof J, de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ Jr, Goedhart J. Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w.)
Proper citation: RRID:Addgene_184570 Copy
Species: Synthetic
Genetic Insert: Cas12c_4
Vector Backbone Description: Vector Backbone:pSC101 ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183071 Copy
Species: Synthetic
Genetic Insert: Cas12c tracrRNA and pre-crRNA
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Comments: A part of the Cas12c2 concatenated noncoding RNA sequence from Addgene plasmid # 120877 (http://n2t.net/addgene:120877 ; RRID:Addgene_120877) was used for tracrRNA expression.
Proper citation: RRID:Addgene_183076 Copy
Species: Synthetic
Genetic Insert: LbCas12a guide RNA containing BbsI sites in spacer sequence
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183222 Copy
Species: Synthetic
Genetic Insert: Cas12c_4
Vector Backbone Description: Vector Backbone:Modified pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35659325
Comments: This Cas12c bacterial expression plasmid was originally created by Harrington et al., 2020 and now modified (R965H) to match with the wild-type protein sequence of Cas12c_4.
Proper citation: RRID:Addgene_183069 Copy
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