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Species: Other
Genetic Insert: HCoV-OC43-nsp1
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168931 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp4
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168932 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp5
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168933 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp6
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168934 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp9
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168937 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp10
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168938 Copy
Species: Other
Genetic Insert: HCoV-NL63-Protein3
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168923 Copy
Species: Other
Genetic Insert: HCoV-NL63-Envelope
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Please note that Addgene identified an IS4-like element transposase in the plasmid backbone. This should not affect Gateway cloning experiments since it is outside of the Gateway cassette.
Proper citation: RRID:Addgene_168924 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp12
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168927 Copy
Species: Other
Genetic Insert: Myc epitope
Vector Backbone Description: Vector Backbone:pHiUGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31272828
Proper citation: RRID:Addgene_200376 Copy
Species: Other
Genetic Insert: V5-epitope
Vector Backbone Description: Vector Backbone:pHiUGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31272828
Proper citation: RRID:Addgene_200378 Copy
Species: Other
Genetic Insert: Myc epitope
Vector Backbone Description: Vector Backbone:pHiUGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31272828
Proper citation: RRID:Addgene_200374 Copy
Species: Other
Genetic Insert: spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
Vector Backbone Description: Vector Backbone:pHiUGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39122707
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.06.511211v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200387 Copy
Species: Other
Genetic Insert: glycosylphosphatidylinositol-Gamillus-Gamillus
Vector Backbone Description: Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37535571
Proper citation: RRID:Addgene_197915 Copy
Species: Other
Genetic Insert: glycosylphosphatidylinositol-mGreenLantern-mGreenLantern
Vector Backbone Description: Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37535571
Proper citation: RRID:Addgene_197917 Copy
Species: Other
Genetic Insert: Plastid transit peptide fused mCherry-UnaG
Vector Backbone Description: Vector Backbone:pGWB602; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37285441
Proper citation: RRID:Addgene_203483 Copy
Species: Other
Genetic Insert: TagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
Vector Backbone Description: Vector Backbone:pGWB511; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37285441
Proper citation: RRID:Addgene_203482 Copy
Species: Other
Genetic Insert: Plastid transit peptide fused UnaG-FLAG
Vector Backbone Description: Vector Backbone:pGWB511; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37285441
Proper citation: RRID:Addgene_203479 Copy
Species: Other
Genetic Insert: V5-epitope
Vector Backbone Description: Vector Backbone:pHiUGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31272828
Proper citation: RRID:Addgene_200379 Copy
Species: Other
Genetic Insert: EGFPnls-barcode206
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pAAV-CAG-tdTomato (codon diversified)(Plasmid #59462); Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38390967
Comments: Please visit https://doi.org/10.1101/2021.05.16.444258 for bioRxiv preprint.
Proper citation: RRID:Addgene_190875 Copy
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