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Species: Synthetic
Genetic Insert: eGFP with Staphylococcus aureus codon optimization
Vector Backbone Description: Backbone Marker:Patrick Trieu-Cuot; Vector Backbone:pAT28; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28264909
Proper citation: RRID:Addgene_120321 Copy
Genetic Insert: g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
Vector Backbone Description: Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30760722
Proper citation: RRID:Addgene_115484 Copy
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10759496
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article.
Kanamycin gene for plant selection on MS media.
Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.
Proper citation: RRID:Addgene_11572 Copy
Species: Arabidopsis thaliana
Genetic Insert: pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_115995 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116013 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116016 Copy
Species: Arabidopsis thaliana
Genetic Insert: pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116010 Copy
Species: Arabidopsis thaliana
Genetic Insert: pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116012 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116006 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSMXL5:GR-LhG4:tSMXL5:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116007 Copy
Species: Arabidopsis thaliana
Genetic Insert: pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116009 Copy
Species: Arabidopsis thaliana
Genetic Insert: pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_115998 Copy
Species: Ricinus communis
Genetic Insert: Ubi10p:RcCHAD-mCherry
Vector Backbone Description: Backbone Size:8913; Vector Backbone:pH7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31133615
Proper citation: RRID:Addgene_127784 Copy
Species: S. pyogenes
Genetic Insert: Sp Cas9
Vector Backbone Description: Backbone Size:7755; Vector Backbone:pCAH01SpR; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30926729
Proper citation: RRID:Addgene_128161 Copy
Vector Backbone Description: Backbone Size:3733; Vector Backbone:pLZ12-Spec; Vector Types:Bacterial mutagenesis; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32430813
Proper citation: RRID:Addgene_128799 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin-like-conjugating enzyme ATG3
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31315929
Proper citation: RRID:Addgene_129293 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin-like-conjugating enzyme ATG3
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31315929
Proper citation: RRID:Addgene_129295 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3B
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31315929
Proper citation: RRID:Addgene_129291 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin-like-conjugating enzyme ATG3
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31315929
Proper citation: RRID:Addgene_129292 Copy
Vector Backbone Description: Backbone Marker:Hellens et al, 2000; Backbone Size:3232; Vector Backbone:pGreen; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32409318
Proper citation: RRID:Addgene_149416 Copy
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