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Species: Caenorhabditis elegans
Genetic Insert: Linker::mKate2::mIAA7
Vector Backbone Description: Backbone Size:2863; Vector Backbone:pBluescript SK (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36029236
Proper citation: RRID:Addgene_188333 Copy
Species: Caenorhabditis elegans
Genetic Insert: mIAA7::mTagBFP2::Linker
Vector Backbone Description: Backbone Size:2863; Vector Backbone:pBluescript SK (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36029236
Proper citation: RRID:Addgene_188334 Copy
Species: Caenorhabditis elegans
Genetic Insert: mIAA7::wrmScarlet::Linker
Vector Backbone Description: Backbone Size:2863; Vector Backbone:pBluescript SK (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36029236
Proper citation: RRID:Addgene_188330 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5::Cas9
Vector Backbone Description: Vector Backbone:pMLS713; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188886 Copy
Species: Caenorhabditis elegans
Genetic Insert: floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
Vector Backbone Description: Vector Backbone:pMLS716; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188889 Copy
Species: Caenorhabditis elegans
Genetic Insert: PU6(R07E5.16)::Ric-4 sgRNA
Vector Backbone Description: Vector Backbone:pBluescript II KS+; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188891 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5::Cas9, Cbr-unc-119
Vector Backbone Description: Vector Backbone:pMLS621; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188892 Copy
Species: Caenorhabditis elegans
Genetic Insert: CTL2 (M222R) of PIEZO1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25242456
Comments: Note: M2225R mutation refers to human mutation of gene. M2092R is mutation in C. elegans.
Proper citation: RRID:Addgene_96952 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ultra-pan-neuronal positive control pUNP::gfp(NLS)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Amp-high-copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: www.wormbuilder.org
MOdular Safe harbor Transgene Insertion (mosTI).
Positive control vector: P_UPN::gfp(NLS)::tbb-2 UTR
Will give unc-119 rescue and neuronal nuclear GFP expression after insertion into any unc-119 mosTI sites.
See Yemini et al. 2021 Cell for development and characterization of the ultra-pan-neuronal promoter.
Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182359 Copy
Species: Caenorhabditis elegans
Genetic Insert: him-8 piRNAi 21ur-1224
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35115715
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159819 Copy
Species: Caenorhabditis elegans
Genetic Insert: array co-injection fragment mosTI Pmlc-2_GFP
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159836 Copy
Species: Caenorhabditis elegans
Genetic Insert: hsf-1
Vector Backbone Description: Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35636511
Proper citation: RRID:Addgene_177786 Copy
Species: Caenorhabditis elegans
Genetic Insert: MCS mosTI NeoR target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159830 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA mosTI neoR
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159831 Copy
Species: Caenorhabditis elegans
Genetic Insert: pDESTR4-R3 mosTI Pmlc-2_GFP target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159842 Copy
Species: Caenorhabditis elegans
Genetic Insert: pDESTR4-R3 mosTI hygroR target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159840 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159829 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA mosTI hygroR
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159827 Copy
Species: Caenorhabditis elegans
Genetic Insert: array co-injection fragment mosTI hygroR
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159828 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159825 Copy
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