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Species: Synthetic
Genetic Insert: Repressor 1E4 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73436 Copy
Species: Synthetic
Genetic Insert: Repressor 3A2 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73435 Copy
Species: Synthetic
Genetic Insert: pAzFRS.2.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Comments: Note: This plasmid exists as a tandem dimer approximately 12 kb in length. Please see the diagnostic digest in the Resource Information section.
Proper citation: RRID:Addgene_73546 Copy
Species: Synthetic
Genetic Insert: pAcFRS.1.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73545 Copy
Species: Synthetic
Genetic Insert: pAcFRS.2.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73544 Copy
Species: Synthetic
Genetic Insert: pAzFRS.1.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73547 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cold temperature germinating 10 (CTG10)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6494; Vector Backbone:pBD-GAL4 Cam; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29632208
Proper citation: RRID:Addgene_67911 Copy
Species: E. coli
Genetic Insert: TraM, TraJ
Vector Backbone Description: Backbone Marker:Registry of Standard Parts; Vector Backbone:pSB3C5; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_67966 Copy
Vector Backbone Description: Backbone Marker:epicentre; Vector Backbone:pCC1FOS; Vector Types:; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_68380 Copy
Species: Synthetic
Genetic Insert: S68TAG beta lactamase
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26350500
Comments: Used to assess phosphoserine or serine incorporation at TAG position corresponding to Ser68 in beta-lactamase protein. Ampicillin resistance only conferred if Ser is incorporated at this position.
Proper citation: RRID:Addgene_68305 Copy
Vector Backbone Description: Backbone Size:5743; Vector Backbone:N/A; Vector Types:Staphylococcal allelic exchange vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22434850
Comments: It is prudent to check that the pIMAY is still temperature sensitive in Staph Aureus before starting any cloning. The author found out that it could lose this function while cultured in Ecoli. That's why Ecoli bearing pIMAY MUST be cultured at 37C in Chloramphenicol (10µg/ml).
Proper citation: RRID:Addgene_68939 Copy
Species: not applicable
Genetic Insert: synthetic circuit
Vector Backbone Description: Vector Backbone:pBAC; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27255669
Proper citation: RRID:Addgene_78214 Copy
Species: not applicable
Genetic Insert: synthetic circuit
Vector Backbone Description: Vector Backbone:pBAC; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27255669
Proper citation: RRID:Addgene_78216 Copy
Species: not applicable
Genetic Insert: synthetic gene circuit
Vector Backbone Description: Vector Backbone:pBAC; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27255669
Proper citation: RRID:Addgene_78220 Copy
Species: not applicable
Genetic Insert: synthetic circuit
Vector Backbone Description: Vector Backbone:pBAC; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27255669
Proper citation: RRID:Addgene_78229 Copy
Genetic Insert: Tet inducible mRFP1
Vector Backbone Description: Backbone Size:2893; Vector Backbone:pSEVA331Bb; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27247386
Proper citation: RRID:Addgene_78283 Copy
Species: Arabidopsis thaliana
Genetic Insert: PAL2
Vector Backbone Description: Backbone Size:2999; Vector Backbone:pSTV28; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21722749
Comments: *Note: Addgene's quality control sequencing finds an E50A amino acid residue substitution in the A. thaliana PAL2 gene insert. The depositing laboratory does not believe this residue change will affect protein function. This plasmid accurately reflects the construct as it was used in the associated publication.
Proper citation: RRID:Addgene_78286 Copy
Genetic Insert: J23117-mRFP1
Vector Backbone Description: Backbone Size:2893; Vector Backbone:pSEVA331Bb; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27247386
Proper citation: RRID:Addgene_78280 Copy
Genetic Insert: J23103-mRFP1
Vector Backbone Description: Backbone Size:2893; Vector Backbone:pSEVA331Bb; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27247386
Proper citation: RRID:Addgene_78273 Copy
Genetic Insert: J23105-mRFP1
Vector Backbone Description: Backbone Size:2893; Vector Backbone:pSEVA331Bb; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27247386
Proper citation: RRID:Addgene_78275 Copy
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