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Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101315 Copy
Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101312 Copy
Species: Mesoplasma florum
Genetic Insert: oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101309 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29164072
Comments: Genotype = ΔompA ΔompC ΔompF ΔlamB
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene.
Due to the strain's growth defect and potential for contamination, Addgene recommends streaking for single colonies from the stab on low salt LB containing tetracycline and then screening colonies by PCR using the Leo Lab's recommended primers to confirm the strain.
Proper citation: RRID:Addgene_102270 Copy
Species: Synthetic
Genetic Insert: PsbA promoter - mTurquoise2
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_108237 Copy
Species: Synthetic
Genetic Insert: PsbA promoter
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_108236 Copy
Species: Synthetic
Genetic Insert: Pc promoter
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_108234 Copy
Vector Backbone Description: Vector Backbone:pAP2; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:18316384
Proper citation: RRID:Addgene_113663 Copy
Vector Backbone Description: Vector Backbone:pL2V-L2-tet-pBBR-ELT3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30692983
Comments: pL2V-L2-tet-pBBR-ELT3. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_114002 Copy
Genetic Insert: Bacteria hybrid selection strain
Vector Backbone Description: Backbone Size:0; Vector Backbone:XL-1 blue F' episome; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16041365
Comments: This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor.
Proper citation: RRID:Addgene_12614 Copy
Species: Z. mobilis
Genetic Insert: pdc, adhB
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20111002
Comments: EJ Steen et al. Nature 2009 10.1038/nature08721
Proper citation: RRID:Addgene_24627 Copy
Species: Z. mobilis, A. baylyi ADP1
Genetic Insert: pdc, adhB, atfA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20111002
Comments: EJ Steen et al. Nature 2009 10.1038/nature08721
Proper citation: RRID:Addgene_24628 Copy
Vector Backbone Description: Backbone Size:5454; Vector Backbone:pBMTBX-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20148414
Proper citation: RRID:Addgene_26075 Copy
Species: synthetic
Genetic Insert: lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:3000; Vector Backbone:pGPS21; Vector Types:in vitro transposion; Bacterial Resistance:Tetracycline
Defining Citation: PMID:15784610
Comments: The plasmid confers Kan resistance (25ug/ml) and Tet resistance (12.5ug/ml). However, it requires pir factor for replication.
Note that this plasmid is supplied on a stab with only Tet. When re-plating, end users should add Kan as well. Also note that the plasmid is only considered high copy in the pir-116 strain. In a WT pir strain, it is considered low copy.
Proper citation: RRID:Addgene_27180 Copy
Species: E. coli
Genetic Insert: MG1655-tMMR
Vector Backbone Description: Vector Backbone:genomic; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24500200
Comments: For detailed use and MAGE, see:
1. Nyerges, Á. et al. Conditional DNA repair mutants enable highly precise genome engineering. Nucl. Acids Res. 42, e62–e62 (2014).
2. Gallagher, R. R., Li, Z., Lewis, A. O. & Isaacs, F. J. Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA. Nat. Protocols 9, 2301–2316 (2014).
3. Bonde, M. T., Anderson, M. V., Wallin, A. I. N., Wang, H. H. & Sommer, M. O. A. MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering. Nucl. Acids Res. (2014). doi:10.1093/nar/gku428
Proper citation: RRID:Addgene_62392 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD4 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69276 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD3 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69275 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD1; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69273 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD6 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69278 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD3 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69286 Copy
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