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Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Slow-growing, requires 3 days growth at 30C
Proper citation: RRID:Addgene_87075 Copy
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26264872
Proper citation: RRID:Addgene_78855 Copy
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26264872
Proper citation: RRID:Addgene_78857 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79021 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79020 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI restriction site.
Please note- the NdeI site has been disrupted in this plasmid. The NdeI site (CATATA instead of CATATG) will generate His-Ile instead of His-Met upon translation to protein. This region is linker between N-tag and protein of interest, so this should not affect protein function.
nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79023 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79025 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79017 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79016 Copy
Genetic Insert: FLAG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pDEST26; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17846994
Proper citation: RRID:Addgene_79275 Copy
Vector Backbone Description: Backbone Marker:cloned in house; Backbone Size:7800; Vector Backbone:pSBDEST.B; Vector Types:Promoterless Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27694838
Comments: Sleeping Beauty compatible, promoterless Gateway Destination Vector, for MultiSite Gateway cloning.
Proper citation: RRID:Addgene_79460 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79469 Copy
Vector Backbone Description: Backbone Marker:cloned in house; Backbone Size:8212; Vector Backbone:pSBDEST.N; Vector Types:Promoterless Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27694838
Comments: Sleeping Beauty compatible, promoterless Gateway Destination Vector, for MultiSite Gateway cloning.
Proper citation: RRID:Addgene_79468 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79465 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79467 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79466 Copy
Vector Backbone Description: Backbone Marker:cloned in house; Backbone Size:7294; Vector Backbone:pSBDEST.IP; Vector Types:Promoterless Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27694838
Comments: Sleeping Beauty compatible, promoterless Gateway Destination Vector, for MultiSite Gateway cloning. Uses an internal ribosomal entry site to tie Puromycin selection to expression of inserted transgene.
Proper citation: RRID:Addgene_79472 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79476 Copy
Vector Backbone Description: Vector Backbone:Binary vector pAMPAT-MCS; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23900543
Proper citation: RRID:Addgene_79755 Copy
Vector Backbone Description: Vector Backbone:Binary vector pAMPAT-MCS; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16311592
Proper citation: RRID:Addgene_79750 Copy
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