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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pMflPT-o4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101315 | oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA) | Mesoplasma florum | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:00:19 | 1 | ||
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pMflT-o4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101312 | oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA) | Mesoplasma florum | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:00:19 | 2 | ||
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pMflT-o1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101309 | oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region) | Mesoplasma florum | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:00:19 | 1 | ||
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BL21ΔABCF Resource Report Resource Website 1+ mentions |
RRID:Addgene_102270 | Tetracycline | PMID:29164072 | Genotype = ΔompA ΔompC ΔompF ΔlamB Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene. Due to the strain's growth defect and potential for contamination, Addgene recommends streaking for single colonies from the stab on low salt LB containing tetracycline and then screening colonies by PCR using the Leo Lab's recommended primers to confirm the strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline | 2026-08-15 01:00:23 | 3 | |||
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pSW002-PpsbA_mTurquoise2 Resource Report Resource Website |
RRID:Addgene_108237 | PsbA promoter - mTurquoise2 | Synthetic | Tetracycline | PMID:29449848 | Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:01:18 | 0 | ||
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pSW002-PpsbA Resource Report Resource Website |
RRID:Addgene_108236 | PsbA promoter | Synthetic | Tetracycline | PMID:29449848 | Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:01:18 | 0 | ||
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pSW002-Pc Resource Report Resource Website |
RRID:Addgene_108234 | Pc promoter | Synthetic | Tetracycline | PMID:29449848 | Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:01:18 | 0 | ||
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pDA12 Resource Report Resource Website |
RRID:Addgene_113663 | Tetracycline | PMID:18316384 | Vector Backbone:pAP2; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:02:14 | 0 | ||||
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pOGG216 Resource Report Resource Website 1+ mentions |
RRID:Addgene_114002 | Tetracycline | PMID:30692983 | pL2V-L2-tet-pBBR-ELT3. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint. | Vector Backbone:pL2V-L2-tet-pBBR-ELT3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | Domesticated for Golden Gate cloning | 2026-08-15 01:02:17 | 1 | ||
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US0hisB-pyrF- Resource Report Resource Website 1+ mentions |
RRID:Addgene_12614 | Bacteria hybrid selection strain | Tetracycline | PMID:16041365 | This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor. | Backbone Size:0; Vector Backbone:XL-1 blue F' episome; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:04:22 | 3 | ||
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pKS13 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24627 | pdc, adhB | Z. mobilis | Tetracycline | PMID:20111002 | EJ Steen et al. Nature 2009 10.1038/nature08721 | Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:12:32 | 1 | |
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pKS17 Resource Report Resource Website |
RRID:Addgene_24628 | pdc, adhB, atfA | Z. mobilis, A. baylyi ADP1 | Tetracycline | PMID:20111002 | EJ Steen et al. Nature 2009 10.1038/nature08721 | Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:12:34 | 0 | |
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pBMTBX-4 Resource Report Resource Website |
RRID:Addgene_26075 | Tetracycline | PMID:20148414 | Backbone Size:5454; Vector Backbone:pBMTBX-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:12:48 | 0 | ||||
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pGPS21loxFRTNeo Resource Report Resource Website |
RRID:Addgene_27180 | lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R | synthetic | Tetracycline | PMID:15784610 | The plasmid confers Kan resistance (25ug/ml) and Tet resistance (12.5ug/ml). However, it requires pir factor for replication. Note that this plasmid is supplied on a stab with only Tet. When re-plating, end users should add Kan as well. Also note that the plasmid is only considered high copy in the pir-116 strain. In a WT pir strain, it is considered low copy. | Backbone Marker:New England Biolabs; Backbone Size:3000; Vector Backbone:pGPS21; Vector Types:in vitro transposion; Bacterial Resistance:Tetracycline | 2026-08-15 01:13:00 | 0 | |
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MG1655-tMMR Resource Report Resource Website |
RRID:Addgene_62392 | MG1655-tMMR | E. coli | Tetracycline | PMID:24500200 | For detailed use and MAGE, see: 1. Nyerges, Á. et al. Conditional DNA repair mutants enable highly precise genome engineering. Nucl. Acids Res. 42, e62–e62 (2014). 2. Gallagher, R. R., Li, Z., Lewis, A. O. & Isaacs, F. J. Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA. Nat. Protocols 9, 2301–2316 (2014). 3. Bonde, M. T., Anderson, M. V., Wallin, A. I. N., Wang, H. H. & Sommer, M. O. A. MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering. Nucl. Acids Res. (2014). doi:10.1093/nar/gku428 | Vector Backbone:genomic; Vector Types:; Bacterial Resistance:Tetracycline | mutS(A134V) mutL(G62S); nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} | 2026-08-15 01:17:52 | 0 |
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pYPQ134A Resource Report Resource Website |
RRID:Addgene_69276 | Tetracycline | PMID:26297141 | Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD4 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline | 2026-08-15 01:18:51 | 0 | ||||
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pYPQ133A Resource Report Resource Website 1+ mentions |
RRID:Addgene_69275 | Tetracycline | PMID:26297141 | Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD3 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline | 2026-08-15 01:18:57 | 1 | ||||
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pYPQ131A Resource Report Resource Website 1+ mentions |
RRID:Addgene_69273 | Tetracycline | PMID:26297141 | Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD1; Vector Types:CRISPR; Bacterial Resistance:Tetracycline | 2026-08-15 01:18:51 | 2 | ||||
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pYPQ136A Resource Report Resource Website |
RRID:Addgene_69278 | Tetracycline | PMID:26297141 | Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD6 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline | 2026-08-15 01:18:57 | 0 | ||||
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pYPQ133C Resource Report Resource Website 1+ mentions |
RRID:Addgene_69286 | Tetracycline | PMID:26297141 | Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD3 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline | 2026-08-15 01:18:51 | 1 |
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