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Species: Caenorhabditis elegans
Genetic Insert: smu-1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDESTR4-R3; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27374334
Comments: MiniMos vector with Psmu-1:smu-1:GFP:smu-1 UTR. Also contains cbr-unc-119 rescue marker. Generated by Gateway cloning.
Proper citation: RRID:Addgene_84826 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans codon optimized GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDESTR4-R3; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27374334
Comments: Expression vector with C. elegans codon-optimized GFP under the eft-3 promoter and with the tbb-2 3'UTR.
Cloned into pCFJ150 for compatibility with mosSCI.
Contains cbr-unc-119 rescue fragment.
Proper citation: RRID:Addgene_84829 Copy
Species: Caenorhabditis elegans
Genetic Insert: 15xUAS-delta pes-10-GFP
Vector Backbone Description: Backbone Marker:Fire Lab C. elegans Vector Kit; Backbone Size:2778; Vector Backbone:pPD117.01; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27992408
Proper citation: RRID:Addgene_85584 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5224; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191444 Copy
Species: Caenorhabditis elegans
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6479; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191447 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6208; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191446 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9939; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191445 Copy
Species: Caenorhabditis elegans
Genetic Insert: Tetanus Toxin Light Chain
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6745; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191372 Copy
Species: Caenorhabditis elegans
Genetic Insert: Arch
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6350; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191477 Copy
Species: Caenorhabditis elegans
Genetic Insert: GCaMP3
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6185; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191358 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mitoguardin homologue
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36282247
Proper citation: RRID:Addgene_192832 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ultra-pan-neuronal positive control pUNP::gfp(NLS)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Amp-high-copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: www.wormbuilder.org
MOdular Safe harbor Transgene Insertion (mosTI).
Positive control vector: P_UPN::gfp(NLS)::tbb-2 UTR
Will give unc-119 rescue and neuronal nuclear GFP expression after insertion into any unc-119 mosTI sites.
See Yemini et al. 2021 Cell for development and characterization of the ultra-pan-neuronal promoter.
Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182359 Copy
Species: Caenorhabditis elegans
Genetic Insert: him-8 piRNAi 21ur-1224
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35115715
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159819 Copy
Species: Caenorhabditis elegans
Genetic Insert: array co-injection fragment mosTI Pmlc-2_GFP
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159836 Copy
Species: Caenorhabditis elegans
Genetic Insert: hsf-1
Vector Backbone Description: Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35636511
Proper citation: RRID:Addgene_177786 Copy
Species: Caenorhabditis elegans
Genetic Insert: MCS mosTI NeoR target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159830 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA mosTI neoR
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159831 Copy
Species: Caenorhabditis elegans
Genetic Insert: pDESTR4-R3 mosTI Pmlc-2_GFP target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159842 Copy
Species: Caenorhabditis elegans
Genetic Insert: pDESTR4-R3 mosTI hygroR target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159840 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159829 Copy
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